Perfusion MRI of U87 Brain Tumors in a Mouse Model

Yanping Sun; Nils O. Schmidt; Karl Schmidt; Sameer Doshi; Joshua B. Rubin; Robert V. Mulkern; Rona Carroll; Mateo Ziu; Kadir Erkmen; Tina Y. Poussaint; Peter Black; Mitchell Albert; Deborah Burstein; Mark W. Kieran

doi:10.1002/mrm.20029

Perfusion MRI of U87 Brain Tumors in a Mouse Model

Yanping Sun, Nils O. Schmidt, Karl Schmidt, Sameer Doshi, Joshua B. Rubin, Robert V. Mulkern, Rona Carroll, Mateo Ziu, Kadir Erkmen, Tina Y. Poussaint, Peter Black, Mitchell Albert, Deborah Burstein, Mark W. Kieran

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Abstract

Continuous arterial spin labeling (CASL) was used to obtain an index of cerebral blood flow (ICBF) in the normal mouse brain and in an orthotopic mouse model of human U87 high-grade glioma at 8.5 T. Under the assumption of a constant tissue: blood partition coefficient for water in different tissues, the mean ICBF (n = 14) was found to be 50 ± 9 mL/100g/min for tumor core and 209 ± 11 mL/100g/min for normal tissue. The apparent T₁ (T_1app) was 2.01 ± 0.06 sec for tumor core and 1.66 ± 0.03 sec for normal tissue. The ICBF and the T_1app values were significantly different (P < 0.001) between these two regions. The detailed changes of ICBF and T_1app in the transition from the tumor core through the tumor periphery to surrounding tissue were studied. Immunohistochemistry indicated that tumor vascularity was not uniform, with microvessel density highest in normal brain and the tissue surrounding the tumor and lowest in the tumor core. The large difference in ICBF between the tumor core and normal tissue suggests that this index might be useful for the assessment of the efficacy of antiangiogenic therapy.

Original language	English
Pages (from-to)	893-899
Number of pages	7
Journal	Magnetic resonance in medicine
Volume	51
Issue number	5
DOIs	https://doi.org/10.1002/mrm.20029
State	Published - May 2004

Keywords

Antiangiogenesis
Arterial spin labeling
Brain tumor in mouse model
Perfusion MRI

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title = "Perfusion MRI of U87 Brain Tumors in a Mouse Model",

abstract = "Continuous arterial spin labeling (CASL) was used to obtain an index of cerebral blood flow (ICBF) in the normal mouse brain and in an orthotopic mouse model of human U87 high-grade glioma at 8.5 T. Under the assumption of a constant tissue: blood partition coefficient for water in different tissues, the mean ICBF (n = 14) was found to be 50 ± 9 mL/100g/min for tumor core and 209 ± 11 mL/100g/min for normal tissue. The apparent T1 (T1app) was 2.01 ± 0.06 sec for tumor core and 1.66 ± 0.03 sec for normal tissue. The ICBF and the T1app values were significantly different (P < 0.001) between these two regions. The detailed changes of ICBF and T1app in the transition from the tumor core through the tumor periphery to surrounding tissue were studied. Immunohistochemistry indicated that tumor vascularity was not uniform, with microvessel density highest in normal brain and the tissue surrounding the tumor and lowest in the tumor core. The large difference in ICBF between the tumor core and normal tissue suggests that this index might be useful for the assessment of the efficacy of antiangiogenic therapy.",

keywords = "Antiangiogenesis, Arterial spin labeling, Brain tumor in mouse model, Perfusion MRI",

author = "Yanping Sun and Schmidt, {Nils O.} and Karl Schmidt and Sameer Doshi and Rubin, {Joshua B.} and Mulkern, {Robert V.} and Rona Carroll and Mateo Ziu and Kadir Erkmen and Poussaint, {Tina Y.} and Peter Black and Mitchell Albert and Deborah Burstein and Kieran, {Mark W.}",

year = "2004",

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doi = "10.1002/mrm.20029",

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T1 - Perfusion MRI of U87 Brain Tumors in a Mouse Model

AU - Sun, Yanping

AU - Schmidt, Nils O.

AU - Schmidt, Karl

AU - Doshi, Sameer

AU - Rubin, Joshua B.

AU - Mulkern, Robert V.

AU - Carroll, Rona

AU - Ziu, Mateo

AU - Erkmen, Kadir

AU - Poussaint, Tina Y.

AU - Black, Peter

AU - Albert, Mitchell

AU - Burstein, Deborah

AU - Kieran, Mark W.

PY - 2004/5

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N2 - Continuous arterial spin labeling (CASL) was used to obtain an index of cerebral blood flow (ICBF) in the normal mouse brain and in an orthotopic mouse model of human U87 high-grade glioma at 8.5 T. Under the assumption of a constant tissue: blood partition coefficient for water in different tissues, the mean ICBF (n = 14) was found to be 50 ± 9 mL/100g/min for tumor core and 209 ± 11 mL/100g/min for normal tissue. The apparent T1 (T1app) was 2.01 ± 0.06 sec for tumor core and 1.66 ± 0.03 sec for normal tissue. The ICBF and the T1app values were significantly different (P < 0.001) between these two regions. The detailed changes of ICBF and T1app in the transition from the tumor core through the tumor periphery to surrounding tissue were studied. Immunohistochemistry indicated that tumor vascularity was not uniform, with microvessel density highest in normal brain and the tissue surrounding the tumor and lowest in the tumor core. The large difference in ICBF between the tumor core and normal tissue suggests that this index might be useful for the assessment of the efficacy of antiangiogenic therapy.

AB - Continuous arterial spin labeling (CASL) was used to obtain an index of cerebral blood flow (ICBF) in the normal mouse brain and in an orthotopic mouse model of human U87 high-grade glioma at 8.5 T. Under the assumption of a constant tissue: blood partition coefficient for water in different tissues, the mean ICBF (n = 14) was found to be 50 ± 9 mL/100g/min for tumor core and 209 ± 11 mL/100g/min for normal tissue. The apparent T1 (T1app) was 2.01 ± 0.06 sec for tumor core and 1.66 ± 0.03 sec for normal tissue. The ICBF and the T1app values were significantly different (P < 0.001) between these two regions. The detailed changes of ICBF and T1app in the transition from the tumor core through the tumor periphery to surrounding tissue were studied. Immunohistochemistry indicated that tumor vascularity was not uniform, with microvessel density highest in normal brain and the tissue surrounding the tumor and lowest in the tumor core. The large difference in ICBF between the tumor core and normal tissue suggests that this index might be useful for the assessment of the efficacy of antiangiogenic therapy.

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KW - Arterial spin labeling

KW - Brain tumor in mouse model

KW - Perfusion MRI

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Perfusion MRI of U87 Brain Tumors in a Mouse Model

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