Perfusion-based fluorescence imaging method delineates diverse organs and identifies multifocal tumors using generic near-infrared molecular probes

Jessica Miller, Steven T. Wang, Inema Orukari, Julie Prior, Gail Sudlow, Xinming Su, Kexian Liang, Rui Tang, Elizabeth M.C. Hillman, Katherine N. Weilbaecher, Joseph P. Culver, Mikhail Y. Berezin, Samuel Achilefu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Rapid detection of multifocal cancer without the use of complex imaging schemes will improve treatment outcomes. In this study, dynamic fluorescence imaging was used to harness differences in the perfusion kinetics of near-infrared (NIR) fluorescent dyes to visualize structural characteristics of different tissues. Using the hydrophobic nontumor-selective NIR dye cypate, and the hydrophilic dye LS288, a high tumor-to-background contrast was achieved, allowing the delineation of diverse tissue types while maintaining short imaging times. By clustering tissue types with similar perfusion properties, the dynamic fluorescence imaging method identified secondary tumor locations when only the primary tumor position was known, with a respective sensitivity and specificity of 0.97 and 0.75 for cypate, and 0.85 and 0.81 for LS288. Histological analysis suggests that the vasculature in the connective tissue that directly surrounds the tumor was a major factor for tumor identification through perfusion imaging. Although the hydrophobic dye showed higher specificity than the hydrophilic probe, use of other dyes with different physical and biological properties could further improve the accuracy of the dynamic imaging platform to identify multifocal tumors for potential use in real-time intraoperative procedures.

Original languageEnglish
Article numbere201700232
JournalJournal of Biophotonics
Volume11
Issue number4
DOIs
StatePublished - Apr 2018

Keywords

  • basis function
  • cancer
  • molecular probe
  • near-infrared
  • optical

Fingerprint Dive into the research topics of 'Perfusion-based fluorescence imaging method delineates diverse organs and identifies multifocal tumors using generic near-infrared molecular probes'. Together they form a unique fingerprint.

  • Cite this