Performance of a no-pretreatment tacrolimus assay on the Dade Behring Dimension RxL clinical chemistry analyzer

Megan A. Griffey, Karl G. Hock, Daniel C. Kilgore, Tie Q. Wei, Show Hong Duh, Robert Christenson, Mitchell G. Scott

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Background: Therapeutic drug monitoring for tacrolimus is important for organ transplant patients receiving this immunosuppressant. Current available assays for tacrolimus require sample pre-treatment and operate in a batch mode. Here a no-pretreatment tacrolimus assay, performed on the Dade Behring Dimension analyzer is compared to the Abbott IMx tacrolimus assay and to an LC/MS/MS method. Methods: Whole blood samples from 2 medical centers and different transplant types (kidney n = 104, liver n = 84, heart n = 28, pancreas n = 16, bone marrow n = 11, lung n = 7), were obtained and tacrolimus quantified by each of the 3 assays. Results: The lower limit of the linear range was 1.2 ng/ml on the Dimension assay. Total imprecision was 9.8% and within-run imprecision was 9.6% at a tacrolimus concentration of 3.4 ng/dL. Passing-Bablock regression analysis determined the following relationships: DIMN = (1.16) LC/MS - 0.43, r = 0.90 and DIMN = (0.99)IMx - 0.35, r = 0.87. Conclusions: The Dade Behring Dimension II Tacrolimus assay has adequate imprecision and correlates well with the reference method of LC/MS/MS. The assay appears suitable for clinical use, and has the advantages of not requiring a pretreatment step and the ability to be performed in a random-access mode.

Original languageEnglish
Pages (from-to)48-51
Number of pages4
JournalClinica Chimica Acta
Volume384
Issue number1-2
DOIs
StatePublished - Sep 2007

Keywords

  • Assay comparison
  • Immunosuppressant drugs
  • Tacrolimus
  • Therapeutic drug monitoring
  • Transplantation

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