Percutaneous muscle biopsy-induced tissue injury causes local endoplasmic reticulum stress

Jun Yoshino, Paloma Almeda-Valdes, Anna C. Moseley, Bettina Mittendorfer, Samuel Klein

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Endoplasmic reticulum (ER) stress is likely involved in the pathogenesis of metabolic dysfunction in people with obesity and diabetes. Although tissue biopsy is often used to evaluate the presence and severity of ER stress, it is not known whether acute tissue injury-induced by percutaneous muscle biopsy causes ER stress and its potential downstream effects on markers of inflammation and metabolic function. In this study, we tested the hypothesis that percutaneous biopsy-induced tissue injury causes ER stress and alters inflammatory and metabolic pathways in skeletal muscle. Vastus lateralis muscle tissue was obtained by percutaneous biopsy at 0600 h and 12 h later from either the contralateral leg (Group 1, n = 6) or at the same site as the initial biopsy (Group 2, n = 6) in women who were overweight. Muscle gene expression of selected markers of ER stress, inflammation, and regulators of glucose and lipid metabolism were determined. Compared with Group 1, muscle gene expression in the second biopsy sample obtained in Group 2 demonstrated marked increases in markers of ER stress (GRP78, XBP1, ATF6) and inflammation (IL6, TNF), and alterations in metabolic regulators (decreased expression of GLUT4 and PPARGC1A and increased expression of FASN). Our results suggest that acute tissue injury induced by percutaneous muscle biopsy causes an integrated local response that involves an induction of ER stress and alterations in markers of inflammation and regulators of glucose and lipid metabolism.

Original languageEnglish
Article numbere13679
JournalPhysiological Reports
Issue number8
StatePublished - Apr 2018


  • ER stress
  • Muscle injury
  • inflammation
  • metabolism
  • percutaneous biopsy


Dive into the research topics of 'Percutaneous muscle biopsy-induced tissue injury causes local endoplasmic reticulum stress'. Together they form a unique fingerprint.

Cite this