Peptidoglycan editing provides immunity to Acinetobacter baumannii during bacterial warfare

Nguyen Hung Le, Katharina Peters, Akbar Espaillat, Jessica R. Sheldon, Joe Gray, Gisela Di Venanzio, Juvenal Lopez, Bardya Djahanschiri, Elizabeth A. Mueller, Seth W. Hennon, Petra Anne Levin, Ingo Ebersberger, Eric P. Skaar, Felipe Cava, Waldemar Vollmer, Mario F. Feldman

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Peptidoglycan (PG) is essential in most bacteria. Thus, it is often targeted by various assaults, including interbacterial attacks via the type VI secretion system (T6SS). Here, we report that the Gram-negative bacterium Acinetobacter baumannii strain ATCC 17978 produces, secretes, and incorporates the noncanonical d-amino acid d-lysine into its PG during stationary phase. We show that PG editing increases the competitiveness of A. baumannii during bacterial warfare by providing immunity against peptidoglycan-targeting T6SS effectors from various bacterial competitors. In contrast, we found that d-Lys production is detrimental to pathogenesis due, at least in part, to the activity of the human enzyme d-amino acid oxidase (DAO), which degrades d-Lys producing H2O2 toxic to bacteria. Phylogenetic analyses indicate that the last common ancestor of A. baumannii had the ability to produce d-Lys. However, this trait was independently lost multiple times, likely reflecting the evolution of A. baumannii as a human pathogen.

Original languageEnglish
Article numberabb5614
JournalScience Advances
Volume6
Issue number30
DOIs
StatePublished - Jul 2020

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    Le, N. H., Peters, K., Espaillat, A., Sheldon, J. R., Gray, J., Venanzio, G. D., Lopez, J., Djahanschiri, B., Mueller, E. A., Hennon, S. W., Levin, P. A., Ebersberger, I., Skaar, E. P., Cava, F., Vollmer, W., & Feldman, M. F. (2020). Peptidoglycan editing provides immunity to Acinetobacter baumannii during bacterial warfare. Science Advances, 6(30), [abb5614]. https://doi.org/10.1126/sciadv.abb5614