Pentose phosphate pathway in cellular trophoblasts from full-term human placentas

A. J. Moe, D. R. Farmer, D. M. Nelson, C. H. Smith

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26 Scopus citations

Abstract

Glucose metabolism was investigated in cellular trophoblasts isolated from full-term human placentas. The specific yields of 14CO2 from D-[1-14C]glucose and D-[6-14C]glucose were used to determine glucose metabolism via the pentose cycle for cells freshly isolated or cells grown in culture for 1 and 3 days. Cells were mononucleated on day 1 but fused to form multinucleated syncytiotrophoblasts by day 3. The principal product of glucose metabolism under all conditions was lactate, accounting for approximately three-fourths of recovered 14C in products. Pentose cycle activity contributed 0.57 ± 0.01, 0.39 ± 0.06, and 0.21 ± 0.05% of the glucose metabolized by cells freshly isolated, cultured for 1 day, and cultured for 3 days, respectively. In the presence of the electron acceptor methylene blue, pentose cycle activity increased to 16.5 ± 2.1, 13.8 ± 1.5, and 18.2 ± 1.7% for cells freshly isolated, cultured for 1 day, and cultured for 3 days, respectively. Trace amounts of 14C were recovered in other products including amino acids and glycogen. These data suggest that pentose cycle activity in cellular trophoblasts from full-term placenta, like those in full-term villous tissue, is a minor component of glucose metabolism. However, these cultured cells maintain a capacity to oxidize glucose via the pentose cycle at relatively high rates.

Original languageEnglish
Pages (from-to)C1042-C1047
JournalAmerican Journal of Physiology - Cell Physiology
Volume261
Issue number6 30-6
DOIs
StatePublished - 1991

Keywords

  • Glucose metabolism
  • Methylene blue
  • Pentose cycle

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