TY - JOUR
T1 - Penetration and activation of brain endothelium by Salmonella enterica serovar typhimurium
AU - Van Sorge, Nina M.
AU - Zialcita, Patricia A.
AU - Browne, Sara H.
AU - Quach, Darin
AU - Guiney, Donald G.
AU - Doran, Kelly S.
N1 - Funding Information:
This work was supported by the National Institute of Neurological Disorders and Stroke/National Institutes of Health (grant AI062758 to S.H.B. and R01NS051247 to K.S.D).
PY - 2011/2/1
Y1 - 2011/2/1
N2 - Salmonella meningitis is a serious disease of the central nervous system, common particularly in Africa. Here, we show that Salmonella enterica serovar Typhimurium is able to adhere, invade, and penetrate human brain microvascular endothelial cells (hBMECs), the single-cell layer constituting the blood-brain barrier (BBB). Cellular invasion was dependent on host actin cytoskeleton rearrangements, while expression of a functional type III secretion system was not essential. In addition, Salmonella infection activated a proinflammatory immune response targeting neutrophil signaling and recruitment. Salmonella invasion and immune activation may represent a crucial step in the penetration of the BBB and development of Salmonella meningitis.
AB - Salmonella meningitis is a serious disease of the central nervous system, common particularly in Africa. Here, we show that Salmonella enterica serovar Typhimurium is able to adhere, invade, and penetrate human brain microvascular endothelial cells (hBMECs), the single-cell layer constituting the blood-brain barrier (BBB). Cellular invasion was dependent on host actin cytoskeleton rearrangements, while expression of a functional type III secretion system was not essential. In addition, Salmonella infection activated a proinflammatory immune response targeting neutrophil signaling and recruitment. Salmonella invasion and immune activation may represent a crucial step in the penetration of the BBB and development of Salmonella meningitis.
UR - http://www.scopus.com/inward/record.url?scp=79751514280&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiq048
DO - 10.1093/infdis/jiq048
M3 - Article
C2 - 21186258
AN - SCOPUS:79751514280
SN - 0022-1899
VL - 203
SP - 401
EP - 405
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 3
ER -