PEA3, AP-1, and a unique repetitive sequence all are involved in transcriptional regulation of the breast cancer-associated gene, mammaglobin

Diane R. Hesselbrock, Natasza Kurpios, John A. Hassell, Mark A. Watson, Timothy P. Fleming

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The breast cancer-associated gene mammaglobin is a member of the secretoglobin protein family and has demonstrated its utility as a breast cancer marker. However, the transcriptional regulation of mammaglobin has not been well-characterized. In this report, we used luciferase reporter assays to identify the 200 bp directly 5' of the transcriptional start site as the minimal promoter region of mammaglobin. Sequence scanning indicated that two PEA3 transcription sites were possibly involved in mammaglobin transcription. By transfecting a PEA3 expression vector into breast cancer cell lines MDA-MB-415 and MCF-7, we determined that exogenous PEA3 was able to drive transcription. Mutational analysis indicated that each PEA3 site was functional. Our reporter system and electrophorectic mobility shift assays (EMSAs) also identified the involvement of a unique repetitive element in mammaglobin transcription. Finally, AP-1 was determined via luciferase assays to be involved in regulating non-PEA3 dependent transcription. Elucidating these cis-acting elements will impact our understanding of transcription of normal breast and breast cancer-associated genes.

Original languageEnglish
Pages (from-to)289-296
Number of pages8
JournalBreast Cancer Research and Treatment
Volume89
Issue number3
DOIs
StatePublished - Feb 2005

Keywords

  • AP-1
  • Breast cancer-associated
  • Gene expression
  • Mammaglobin
  • PEA3

Fingerprint

Dive into the research topics of 'PEA3, AP-1, and a unique repetitive sequence all are involved in transcriptional regulation of the breast cancer-associated gene, mammaglobin'. Together they form a unique fingerprint.

Cite this