@article{7b3a0e580aea4a0eb7020fbee3cf8d46,
title = "PDF receptor signaling in Drosophila contributes to both circadian and geotactic behaviors",
abstract = "The neuropeptide Pigment-Dispersing Factor (PDF) is a principle transmitter regulating circadian locomotor rhythms in Drosophila. We have identified a Class II (secretin-related) G protein-coupled receptor (GPCR) that is specifically responsive to PDF and also to calcitonin-like peptides and to PACAP. In response to PDF, the PDF receptor (PDFR) elevates cAMP levels when expressed in HEK293 cells. As predicted by in vivo studies, cotransfection of Neurofibromatosis Factor 1 significantly improves coupling of PDFR to adenylate cyclase. pdfr mutant flies display increased circadian arrhythmicity, and also display altered geotaxis that is epistatic to that of pdf mutants. PDFR immunosignals are expressed by diverse neurons, but only by a small subset of circadian pacemakers. These data establish the first synapse within the Drosophila circadian neural circuit and underscore the importance of Class II peptide GPCR signaling in circadian neural systems.",
author = "Inge Mertens and Anick Vandingenen and Johnson, \{Erik C.\} and Shafer, \{Orie T.\} and W. Li and Trigg, \{J. S.\} and \{De Loof\}, Arnold and Liliane Schoofs and Taghert, \{Paul H.\}",
note = "Funding Information: We thank Jan Veenstra, Michael Adams, Ian Dickerson, Julian Dow, David Schooley, and Scott Waddell, Ulrike Heberlein, Michael Rosbash, Gerd Gade, Andre Bernards, David Guttman, Jae Park, and Alan Kopin, for sharing reagents and information; the Bloomington Stock Center and Baylor P element Project, for fly stocks; and the Berkeley Drosophila Genome Project, for genomic information. We thank L. Vanden Bosch, C. Collard, and S. Van Soest, for excellent technical assistance, and Seol Hee Im, for participating in genetic experiments. We thank Ravi Allada and his colleagues for sharing information and unpublished results. We thank Erik Herzog, Joel Levine, and Russ Van Gelder for comments on the manuscript. I.M. was supported by the IWT Flanders, and A.V., by the Research Foundation K.U. Leuven. E.C.J. was supported by a Keck Fellowship and an NRSA fellowship (NS56376). O.T.S. was supported by a Keck Fellowship and by a NIH Vision Training Grant. The work was supported by the Flemish Science Foundation (FWO G.0175.02, G146.03, and G0444.05) to L.S., and by grants from the Human Frontier Scientific Program Organization and the NIH (NS27149 and MH067122) to P.H.T. ",
year = "2005",
month = oct,
day = "20",
doi = "10.1016/j.neuron.2005.09.009",
language = "English",
volume = "48",
pages = "213--219",
journal = "Neuron",
issn = "0896-6273",
number = "2",
}