PDF receptor signaling in Drosophila contributes to both circadian and geotactic behaviors

Inge Mertens, Anick Vandingenen, Erik C. Johnson, Orie T. Shafer, W. Li, J. S. Trigg, Arnold De Loof, Liliane Schoofs, Paul H. Taghert

Research output: Contribution to journalArticle

231 Scopus citations

Abstract

The neuropeptide Pigment-Dispersing Factor (PDF) is a principle transmitter regulating circadian locomotor rhythms in Drosophila. We have identified a Class II (secretin-related) G protein-coupled receptor (GPCR) that is specifically responsive to PDF and also to calcitonin-like peptides and to PACAP. In response to PDF, the PDF receptor (PDFR) elevates cAMP levels when expressed in HEK293 cells. As predicted by in vivo studies, cotransfection of Neurofibromatosis Factor 1 significantly improves coupling of PDFR to adenylate cyclase. pdfr mutant flies display increased circadian arrhythmicity, and also display altered geotaxis that is epistatic to that of pdf mutants. PDFR immunosignals are expressed by diverse neurons, but only by a small subset of circadian pacemakers. These data establish the first synapse within the Drosophila circadian neural circuit and underscore the importance of Class II peptide GPCR signaling in circadian neural systems.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalNeuron
Volume48
Issue number2
DOIs
StatePublished - Oct 20 2005

Fingerprint Dive into the research topics of 'PDF receptor signaling in Drosophila contributes to both circadian and geotactic behaviors'. Together they form a unique fingerprint.

  • Cite this

    Mertens, I., Vandingenen, A., Johnson, E. C., Shafer, O. T., Li, W., Trigg, J. S., De Loof, A., Schoofs, L., & Taghert, P. H. (2005). PDF receptor signaling in Drosophila contributes to both circadian and geotactic behaviors. Neuron, 48(2), 213-219. https://doi.org/10.1016/j.neuron.2005.09.009