PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris

John W. Sumner, Gregory A. Storch, Richard S. Buller, Allison M. Liddell, Steven L. Stockham, Yasuko Rikihisa, Sharon Messenger, Christopher D. Paddock

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

Original languageEnglish
Pages (from-to)2746-2749
Number of pages4
JournalJournal of clinical microbiology
Volume38
Issue number7
StatePublished - Aug 7 2000

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