PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris

John W. Sumner; Gregory A. Storch; Richard S. Buller; Allison M. Liddell; Steven L. Stockham; Yasuko Rikihisa; Sharon Messenger; Christopher D. Paddock

doi:10.1128/jcm.38.7.2746-2749.2000

PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris

John W. Sumner, Gregory A. Storch, Richard S. Buller, Allison M. Liddell, Steven L. Stockham, Yasuko Rikihisa, Sharon Messenger, Christopher D. Paddock

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

Original language	English
Pages (from-to)	2746-2749
Number of pages	4
Journal	Journal of clinical microbiology
Volume	38
Issue number	7
DOIs	https://doi.org/10.1128/jcm.38.7.2746-2749.2000
State	Published - 2000

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10.1128/jcm.38.7.2746-2749.2000

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title = "PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris",

abstract = "Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.",

author = "Sumner, {John W.} and Storch, {Gregory A.} and Buller, {Richard S.} and Liddell, {Allison M.} and Stockham, {Steven L.} and Yasuko Rikihisa and Sharon Messenger and Paddock, {Christopher D.}",

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AU - Sumner, John W.

AU - Storch, Gregory A.

AU - Buller, Richard S.

AU - Liddell, Allison M.

AU - Stockham, Steven L.

AU - Rikihisa, Yasuko

AU - Messenger, Sharon

AU - Paddock, Christopher D.

PY - 2000

Y1 - 2000

N2 - Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

AB - Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

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U2 - 10.1128/jcm.38.7.2746-2749.2000

DO - 10.1128/jcm.38.7.2746-2749.2000

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AN - SCOPUS:0033942266

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JO - Journal of clinical microbiology

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IS - 7

ER -

PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris

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