Pcdhαc2 is required for axonal tiling and assembly of serotonergic circuitries in mice

Weisheng V. Chen, Chiamaka L. Nwakeze, Christine A. Denny, Sean O'Keeffe, Michael A. Rieger, George Mountoufaris, Amy Kirner, Joseph D. Dougherty, René Hen, Qiang Wu, Tom Maniatis

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Serotonergic neurons project their axons pervasively throughout the brain and innervate various target fields in a space-filling manner, leading to tiled arrangements of their axon terminals to allow optimal allocation of serotonin among target neurons. Here we show that conditional deletion of the mouse protocadherin α (Pcdhα) gene cluster in serotonergic neurons disrupts local axonal tiling and global assembly of serotonergic circuitries and results in depression-like behaviors. Genetic dissection and expression profiling revealed that this role is specifically mediated by Pcdhαc2, which is the only Pcdhα isoform expressed in serotonergic neurons. We conclude that, in contrast to neurite self-avoidance, which requires single-cell identity mediated by Pcdh diversity, a single cell-type identity mediated by the common C-type Pcdh isoform is required for axonal tiling and assembly of serotonergic circuitries.

Original languageEnglish
Pages (from-to)406-411
Number of pages6
JournalScience
Volume356
Issue number6336
DOIs
StatePublished - Apr 28 2017

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