Pathogen reduction in human plasma using an ultrashort pulsed laser

Shaw Wei D. Tsen, David H. Kingsley, Karen Kibler, Bert Jacobs, Sara Sizemore, Sara M. Vaiana, Jeanne Anderson, Kong Thon Tsen, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus, and they introduce chemicals with concerns of side effects which prevent their widespread use. In this report, we demonstrate the inactivation of both enveloped and non-enveloped viruses in human plasma using a novel chemical-free method, a visible ultrashort pulsed laser. We found that laser treatment resulted in 2-log, 1-log, and 3-log reductions in human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus in human plasma, respectively. Laser-treated plasma showed ≥70% retention for most coagulation factors tested. Furthermore, laser treatment did not alter the structure of a model coagulation factor, fibrinogen. Ultrashort pulsed lasers are a promising new method for chemical-free, broad-spectrum pathogen reduction in human plasma.

Original languageEnglish
Article numbere111673
JournalPloS one
Volume9
Issue number11
DOIs
StatePublished - Nov 5 2014

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