TY - JOUR
T1 - Partial purification of a novel mitogen for oligodendroglia
AU - Kreider, Barbara Q.
AU - Grinspan, J. B.
AU - Waterstone, M. B.
AU - Bramblett, G. T.
AU - Ances, B.
AU - Williams, M.
AU - Stern, J.
AU - Lee, V. M.‐Y
AU - Pleasure, D.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - A protein with a MWapp of 50–70 kDa isolated from the salt extract of crude membranes from neonatal rat brain increases the numbers of oligodendroglia in mixed glial cultures prepared from neonatal rat cerebral white matter. After partial purification by ion exchange and gel exclusion chromatography, and elution from an SDS‐polyacrylamide gel, this protein ( “oligodendroglial trophic factor,” OTF) elicited half‐maximal oligodendroglial recruitment at a concentration of 5 ng/mL. OTF is a mitogen for oligodendroglia, and to a lesser extent, for oligodendroglial progenitor (O2A) cells, but does not stimulate proliferation of astroglia, Schwann cells, or endoneurial fibroblasts. OTF, unlike platelet‐derived growth factor (PDGF), is not an oligodendroglial survival factor. Antibodies against PDGF and basic fibroblast growth factor (bFGF) do not interfere with the accumulation of oligodendroglia induced by OTF. When OTF is given simultaneously with either PDGF or bFGF, there is an additive increase in the numbers of cells of the oligodendroglial lineage. © 1995 Wiley‐Liss, Inc.
AB - A protein with a MWapp of 50–70 kDa isolated from the salt extract of crude membranes from neonatal rat brain increases the numbers of oligodendroglia in mixed glial cultures prepared from neonatal rat cerebral white matter. After partial purification by ion exchange and gel exclusion chromatography, and elution from an SDS‐polyacrylamide gel, this protein ( “oligodendroglial trophic factor,” OTF) elicited half‐maximal oligodendroglial recruitment at a concentration of 5 ng/mL. OTF is a mitogen for oligodendroglia, and to a lesser extent, for oligodendroglial progenitor (O2A) cells, but does not stimulate proliferation of astroglia, Schwann cells, or endoneurial fibroblasts. OTF, unlike platelet‐derived growth factor (PDGF), is not an oligodendroglial survival factor. Antibodies against PDGF and basic fibroblast growth factor (bFGF) do not interfere with the accumulation of oligodendroglia induced by OTF. When OTF is given simultaneously with either PDGF or bFGF, there is an additive increase in the numbers of cells of the oligodendroglial lineage. © 1995 Wiley‐Liss, Inc.
KW - growth factor
KW - lineage
KW - mitogen
KW - oligodendroglia
KW - oligodendroglial trophic factor
UR - http://www.scopus.com/inward/record.url?scp=0028893266&partnerID=8YFLogxK
U2 - 10.1002/jnr.490400106
DO - 10.1002/jnr.490400106
M3 - Article
C2 - 7714925
AN - SCOPUS:0028893266
SN - 0360-4012
VL - 40
SP - 44
EP - 53
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 1
ER -