Partial purification and characterization of a factor from a cloned thymic epithelium cell line

J. D. Pfeifer, G. D. Wetzel, R. W. Dutton

Research output: Contribution to journalArticlepeer-review

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Abstract

The culture supernatant from a cloned line of thymic epithelium (TEPI) is shown to enhance the response of thymocytes to alloantigen as measured by cell-mediated lympholysis. The supernatant has no effect on the spleen cell response to alloantigen as measured by cell-mediated lysis and does not contain interleukin 1, interleukin 2, interleukin 3, or interferon-γ activity. The activity is shown to have an apparent m.w. of 160,000 by Sephacryl S-200 gel permeation chromatography, to have an isoelectric point of 6.5, and to elute from DEAE-Sepharose at 0.07 M NaCl.

Original languageEnglish
Pages (from-to)555-561
Number of pages7
JournalJournal of Immunology
Volume136
Issue number2
StatePublished - 1986

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