Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit

Keith E. Isenberg, Imeine A. Ukhun, Sheldon G. Holstad, Samir Jafri, Uchiro Uchida, Charles F. Zorumski, Jay Yang

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Nerve growth factor (NGF) stimulates 5-HT3 receptor expression although the mechanism(s) responsible for this effect are unknown. We report the nucleotide and deduced amino acid sequences of a nearly complete rat 5-HT3 receptor subunit and use the sequence to develop a reverse transcription-polymerase chain reaction (RT-PCR) assay that measures 5-HT3 mRNA. Exposure of PC12 cells to NGF results in increasing steady-state levels of 5-HT3 mRNA. The four-fold increase in mRNA correlates with and is sufficient to account for increases in receptor measured by agonist induction of whole cell currents.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalNeuroReport
Volume5
Issue number2
DOIs
StatePublished - Nov 1993

Keywords

  • 5-HT receptor
  • Nerve growth factor
  • PC12 cells
  • Posttranscriptional effects
  • Steady-state mRNA levels

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