Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit

Keith E. Isenberg; Imeine A. Ukhun; Sheldon G. Holstad; Samir Jafri; Uchiro Uchida; Charles F. Zorumski; Jay Yang

doi:10.1097/00001756-199311180-00006

Partial cDNA cloning and NGF regulation of a rat 5-HT₃ receptor subunit

Keith E. Isenberg, Imeine A. Ukhun, Sheldon G. Holstad, Samir Jafri, Uchiro Uchida, Charles F. Zorumski, Jay Yang

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54 Scopus citations

Abstract

Nerve growth factor (NGF) stimulates 5-HT₃ receptor expression although the mechanism(s) responsible for this effect are unknown. We report the nucleotide and deduced amino acid sequences of a nearly complete rat 5-HT₃ receptor subunit and use the sequence to develop a reverse transcription-polymerase chain reaction (RT-PCR) assay that measures 5-HT₃ mRNA. Exposure of PC12 cells to NGF results in increasing steady-state levels of 5-HT₃ mRNA. The four-fold increase in mRNA correlates with and is sufficient to account for increases in receptor measured by agonist induction of whole cell currents.

Original language	English
Pages (from-to)	121-124
Number of pages	4
Journal	NeuroReport
Volume	5
Issue number	2
DOIs	https://doi.org/10.1097/00001756-199311180-00006
State	Published - Nov 1993

Keywords

5-HT receptor
Nerve growth factor
PC12 cells
Posttranscriptional effects
Steady-state mRNA levels

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10.1097/00001756-199311180-00006

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abstract = "Nerve growth factor (NGF) stimulates 5-HT3 receptor expression although the mechanism(s) responsible for this effect are unknown. We report the nucleotide and deduced amino acid sequences of a nearly complete rat 5-HT3 receptor subunit and use the sequence to develop a reverse transcription-polymerase chain reaction (RT-PCR) assay that measures 5-HT3 mRNA. Exposure of PC12 cells to NGF results in increasing steady-state levels of 5-HT3 mRNA. The four-fold increase in mRNA correlates with and is sufficient to account for increases in receptor measured by agonist induction of whole cell currents.",

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AU - Holstad, Sheldon G.

AU - Jafri, Samir

AU - Uchida, Uchiro

AU - Zorumski, Charles F.

AU - Yang, Jay

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AB - Nerve growth factor (NGF) stimulates 5-HT3 receptor expression although the mechanism(s) responsible for this effect are unknown. We report the nucleotide and deduced amino acid sequences of a nearly complete rat 5-HT3 receptor subunit and use the sequence to develop a reverse transcription-polymerase chain reaction (RT-PCR) assay that measures 5-HT3 mRNA. Exposure of PC12 cells to NGF results in increasing steady-state levels of 5-HT3 mRNA. The four-fold increase in mRNA correlates with and is sufficient to account for increases in receptor measured by agonist induction of whole cell currents.

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KW - Steady-state mRNA levels

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Partial cDNA cloning and NGF regulation of a rat 5-HT₃ receptor subunit

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