TY - JOUR
T1 - Pancreatic lipase-related protein 2 but not classical pancreatic lipase hydrolyzes galactolipids
AU - Andersson, Lena
AU - Carrière, Frédéric
AU - Lowe, Mark E.
AU - Nilsson, Åke
AU - Verger, Robert
N1 - Funding Information:
This researchw as supportebdy grantsf rom the Swedish Medical ResearchC ouncil (No. 3969), The Albert P~hls- son FoundationS, cotiaLipidTeknik AB and the Medical Faculty, Universityo f Lund; also by the EuropeanC om-munitiesi n the frameworko f the BIOTECH programme (BIO2-CT943041), National Institutes of Health Grant DK-33487. This work was done during the tenureo f an EstablishedIn vestigatorsh(ipM .E,L.) from the American Heart Association.
PY - 1996/8/16
Y1 - 1996/8/16
N2 - The pancreatic lipase family contains three subfamilies, the 'classical' lipases and the pancreatic lipase-related proteins 1 (PLRP1) and 2 (PLRP2). Galactolipids are present in membranes of leaves and vegetables and consist of digalactosyldiacylglycerol (DGalDG) monogalactosyldiacylglycerol (MGalDG) and sulfoquinovosyldiacylglycerol (SQDG). These lipids were incubated with PLRP2 from guinea-pig (GPLRP2) and rat (RPLRP2). In the presence of bile salts DGalDG was efficiently hydrolyzed by GPLRP2 and, although less efficiently, by RPLRP2 to digalactosylmonoacylglycerol (DGalMG), free fatty acids and water-soluble galactose-containing compounds. Also, MGalDG and SQDG were hydrolyzed by GPLRP2 and RPLRP2. These data suggest a possible role of PLRP2 in the digestion of dietary galactolipids.
AB - The pancreatic lipase family contains three subfamilies, the 'classical' lipases and the pancreatic lipase-related proteins 1 (PLRP1) and 2 (PLRP2). Galactolipids are present in membranes of leaves and vegetables and consist of digalactosyldiacylglycerol (DGalDG) monogalactosyldiacylglycerol (MGalDG) and sulfoquinovosyldiacylglycerol (SQDG). These lipids were incubated with PLRP2 from guinea-pig (GPLRP2) and rat (RPLRP2). In the presence of bile salts DGalDG was efficiently hydrolyzed by GPLRP2 and, although less efficiently, by RPLRP2 to digalactosylmonoacylglycerol (DGalMG), free fatty acids and water-soluble galactose-containing compounds. Also, MGalDG and SQDG were hydrolyzed by GPLRP2 and RPLRP2. These data suggest a possible role of PLRP2 in the digestion of dietary galactolipids.
KW - Digalactosyldiacylglycerol
KW - Digestion
KW - Galactolipid
KW - Monogalactosyldiacylglycerol
KW - Pancreatic lipase-related protein
KW - Sulfoquinovosyldiacyl-glycerol
UR - http://www.scopus.com/inward/record.url?scp=0000392201&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(96)00068-9
DO - 10.1016/0005-2760(96)00068-9
M3 - Article
C2 - 8765145
AN - SCOPUS:0000392201
SN - 0005-2760
VL - 1302
SP - 236
EP - 240
JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
IS - 3
ER -