TY - JOUR
T1 - P53 gene mutations in non-Hodgkin's lymphoma.
AU - Chen, Y.
AU - Xiang, Z.
AU - Li, H.
AU - Yang, N.
AU - Zhang, H.
N1 - Funding Information:
The samples embedded with paraffin from 29 cases of non-Hodgkin's lymphoma (NHL) were studied from Jan. 1994 to June 1996. The subjects included 21 males and 8 CHEN Yan, male, born in 1952, Professor 9 This project was supported by national initiative programs for scholars coming back from other countries (No. 95-135).
PY - 1999
Y1 - 1999
N2 - To understand P53 gene change of non-Hodgkin's lymphoma (NHL) and human malignant lymphoma cell lines, the exons 5-7 of 29 patients with NHL and 9 kinds of human malignant lymphoma cell lines were studied by silver staining PCR-SSCP technique. Three cases of P53 gene point mutation was found in 29 cases of NHL. Mutation developed in exon 5 in 2 cases, and in exon 6 in 1 case. They were all diffuse lymphoma. In mutation cases, B-cell lymphoma accounted for 2 cases and the other one was T-cell lymphoma. There was no P53 gene mutation in low-grade follicular lymphoma. Seven strains out of 9 kinds of lymphoma cell lines had P53 gene point mutation. One strain had the mutation in exon 5; 5 strains in exon 6 and 1 strain in exons 5, 6, 7. There was a high mutation rate in lymphoma cell lines and low mutation rate in NHL patients. P53 gene plays an important role in lymphoma cell line establishment, cell regeneration and disease evolution.
AB - To understand P53 gene change of non-Hodgkin's lymphoma (NHL) and human malignant lymphoma cell lines, the exons 5-7 of 29 patients with NHL and 9 kinds of human malignant lymphoma cell lines were studied by silver staining PCR-SSCP technique. Three cases of P53 gene point mutation was found in 29 cases of NHL. Mutation developed in exon 5 in 2 cases, and in exon 6 in 1 case. They were all diffuse lymphoma. In mutation cases, B-cell lymphoma accounted for 2 cases and the other one was T-cell lymphoma. There was no P53 gene mutation in low-grade follicular lymphoma. Seven strains out of 9 kinds of lymphoma cell lines had P53 gene point mutation. One strain had the mutation in exon 5; 5 strains in exon 6 and 1 strain in exons 5, 6, 7. There was a high mutation rate in lymphoma cell lines and low mutation rate in NHL patients. P53 gene plays an important role in lymphoma cell line establishment, cell regeneration and disease evolution.
UR - https://www.scopus.com/pages/publications/0142090620
U2 - 10.1007/bf02895589
DO - 10.1007/bf02895589
M3 - Article
C2 - 12840870
AN - SCOPUS:0142090620
SN - 0257-716X
VL - 19
SP - 27
EP - 30
JO - Journal of Tongji Medical University = Tong ji yi ke da xue xue bao
JF - Journal of Tongji Medical University = Tong ji yi ke da xue xue bao
IS - 1
ER -