P-SAMS: A web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design

Noah Fahlgren; Steven T. Hill; James C. Carrington; Alberto Carbonell

doi:10.1093/bioinformatics/btv534

P-SAMS: A web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design

Noah Fahlgren
, Steven T. Hill
, James C. Carrington
, Alberto Carbonell

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors. Availability and implementation: The P-SAMS website is available at http://p-sams.carringtonlab.org.

Original language	English
Pages (from-to)	157-158
Number of pages	2
Journal	Bioinformatics
Volume	32
Issue number	1
DOIs	https://doi.org/10.1093/bioinformatics/btv534
State	Published - Jan 1 2016

Access to Document

10.1093/bioinformatics/btv534

Cite this

@article{36dc51c68e664f958b4ae6963690e5ba,

title = "P-SAMS: A web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design",

abstract = "Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors. Availability and implementation: The P-SAMS website is available at http://p-sams.carringtonlab.org.",

author = "Noah Fahlgren and Hill, \{Steven T.\} and Carrington, \{James C.\} and Alberto Carbonell",

note = "Publisher Copyright: {\textcopyright} 2015 The Author 2015. Published by Oxford University Press.",

year = "2016",

month = jan,

day = "1",

doi = "10.1093/bioinformatics/btv534",

language = "English",

volume = "32",

pages = "157--158",

journal = "Bioinformatics",

issn = "1367-4803",

number = "1",

}

TY - JOUR

T1 - P-SAMS

T2 - A web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design

AU - Fahlgren, Noah

AU - Hill, Steven T.

AU - Carrington, James C.

AU - Carbonell, Alberto

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors. Availability and implementation: The P-SAMS website is available at http://p-sams.carringtonlab.org.

AB - Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors. Availability and implementation: The P-SAMS website is available at http://p-sams.carringtonlab.org.

UR - https://www.scopus.com/pages/publications/84959922714

U2 - 10.1093/bioinformatics/btv534

DO - 10.1093/bioinformatics/btv534

M3 - Article

C2 - 26382195

AN - SCOPUS:84959922714

SN - 1367-4803

VL - 32

SP - 157

EP - 158

JO - Bioinformatics

JF - Bioinformatics

IS - 1

ER -

P-SAMS: A web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design

Abstract

Access to Document

Other files and links

Fingerprint

Cite this