Oxygen-17 appears only in protein in water-stressed soybean leaves labeled by 17O2

Terry Gullion, Tsyr Yan Yu, Manmilan Singh, Gary J. Patti, Gregory S. Potter, Jacob Schaefer

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


We have used a rotational-echo adiabatic-passage double-resonance 13C{17O} solid-state NMR experiment to prove that the glycine produced in the oxygenase reaction of ribulose bisphosphate carboxylase-oxygenase is incorporated exclusively into protein (or protein precursors) of intact, water-stressed soybean leaves exposed to 13CO2 and 17O2. The water stress increased stomatal resistance and decreased gas exchange so that the Calvin cycle in the leaf chloroplasts was no more than 35% 13C isotopically enriched. Labeled O2 levels were sufficient, however, to increase the 17O isotopic concentration of oxygenase products 20-fold over the natural-abundance level of 0.04%. The observed direct incorporation of glycine into protein shows that water stress suppresses photorespiration in soybean leaves.

Original languageEnglish
Pages (from-to)10802-10807
Number of pages6
JournalJournal of the American Chemical Society
Issue number31
StatePublished - Aug 11 2010


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