Oxidative stress mediates synthesis of cytosolic phospholipase a 2 after UVB injury

Xi Chen, Alane Gresham, Aubrey Morrison, Alice P. Pentland

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68 Scopus citations


UVB irradiation has previously been shown to significantly increase phospholipase activity and prostaglandin synthesis. Because UVB irradiation is a potent oxidative stress, the role of active oxygen species in regulating UV-induced cPLA2 synthesis and phosphorylation was examined. In the present study, irradiation produced a 3-fold increase in synthesis within 6 h following irradiation. Phosphorylation of cPLA2 was also increased to a similar extent. UVB-induced synthesis and phosphorylation of cPLA2 could be inhibited by pretreatment with the antioxidants 2,2,5,7,8-pentamethyl-6-hydroxychromane (50 ^iM) or W-acetylcysteine (10 mM). Treatment of unirradiated cultures with the potent oxidant tert-buty\ hydroperoxide (500 /jiM) also increased cPLA2 synthesis and phosphorylation, suggesting that oxidative injury is an important regulator of cPLA2 synthesis. Increased synthesis of cPLA2 correlated well with increased [3H]arachidonic acid release, PGE2 synthesis and lipid peroxidation in epidermis after oxidant or UVB treatment. The results indicate that UVB-induced upregulation of cPLA -, synthesis is mediated by UVB-induced formation of free radicals. I

Original languageEnglish
Pages (from-to)23-33
Number of pages11
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Issue number1
StatePublished - 1996


  • Keratinocyte
  • Oxidative stress
  • Phospholipase a2, cytosolic
  • Prostaglandin
  • Synthesis
  • Ultraviolet light


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