OVOL2 is a critical regulator of ER71/ETV2 in generating FLK1+, hematopoietic, and endothelial cells from embryonic stem cells

Ju Young Kim, Ra Ham Lee, Tae Min Kim, Dong Wook Kim, Young Joo Jeon, Sung Ho Huh, Se Yeong Oh, Michael Kyba, Hiroshi Kataoka, Kyunghee Choi, David M. Ornitz, Jung Il Chae, Changwon Park

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15 Scopus citations

Abstract

In this study, we report that OVOL2, a C2H2zinc finger protein, is a novel binding protein of ER71, which is a critical transcription factor for blood and vessel development. OVOL2 directly interacted with ER71, but not with ETS1 or ETS2, in the nucleus. ER71-mediated activation of the Flk1 promoter was further enhanced by OVOL2, although OVOL2 alone failed to activate it. Consistently, coexpression of ER71 and OVOL2 in differentiating embryonicstem cells ledtoasignificant augmentation of FLK1+, endothelial, and hematopoietic cells. Such cooperative effects were impaired by the short hairpin RNA-mediated inhibition of Ovol2. Collectively, weshow that ER71 directly interacts with OVOL2 and that such interaction is critical for FLK1+ cell generation and their differentiation into downstream cell lineages.

Original languageEnglish
Pages (from-to)2948-2952
Number of pages5
JournalBlood
Volume124
Issue number19
DOIs
StatePublished - Nov 6 2014

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    Kim, J. Y., Lee, R. H., Kim, T. M., Kim, D. W., Jeon, Y. J., Huh, S. H., Oh, S. Y., Kyba, M., Kataoka, H., Choi, K., Ornitz, D. M., Chae, J. I., & Park, C. (2014). OVOL2 is a critical regulator of ER71/ETV2 in generating FLK1+, hematopoietic, and endothelial cells from embryonic stem cells. Blood, 124(19), 2948-2952. https://doi.org/10.1182/blood-2014-03-556332