Overexpression of the SK3 channel alters vascular remodeling during pregnancy, leading to fetal demise

Cara C. Rada, Stephanie L. Pierce, Daniel W. Nuno, Kathy Zimmerman, Kathryn G. Lamping, Noelle C. Bowdler, Robert M. Weiss, Sarah K. England

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The maternal cardiovascular system undergoes hemodynamic changes during pregnancy via angiogenesis and vasodilation to ensure adequate perfusion of the placenta. Improper vascularization at the maternalfetal interface can cause pregnancy complications and poor fetal outcomes. Recent evidence indicates that small conductance Ca2+ - activated K+ channel subtype 3 (SK3) contributes to vascular remodeling during pregnancy, and we hypothesized that abnormal SK3 channel expression would alter the ability of the maternal cardiovascular system to adapt to pregnancy demands and lead to poor fetal outcomes. We investigated this hypothesis using transgenic Kcnn3tm 1Jpad/Kcnn3tm 1Jpad (SK3T/T) mice that overexpress the channel. Isolated pressurized uterine arteries from nonpregnant transgenic SK3T/T mice had larger basal diameters and decreased agonist-induced constriction than those from their wild-type counterparts; however, non-receptor-mediated depolarization remained intact. In addition to vascular changes, heart rates and ejection fraction were increased, whereas end systolic volume was reduced in SK3T/T mice compared with their wild-type littermates. Uterine sonography of the fetuses on pregnancy day 14 showed a significant decrease in fetal size in SK3T/T compared with wild-type mice; thus, SK3T/T mice displayed an intrauterine growth-restricted phenotype. The SK3T/T mice showed decreased placental thicknesses and higher incidence of fetal loss, losing over half of their complement of pups by midgestation. These results establish that the SK3 channel contributes to both maternal and fetal outcomes during pregnancy and point to the importance of SK3 channel regulation in maintaining a healthy pregnancy.

Original languageEnglish
Pages (from-to)E825-E831
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume303
Issue number7
DOIs
StatePublished - Oct 1 2012

Keywords

  • Pregnancy
  • Small conductance Caactivated K channel subtype 3
  • Vasculature

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