Abstract
In order to investigate the role of Bcl-2 in dopaminergic cells, we established a dopaminergic neuronal cell line (MN9D) stably expressing human Bcl-2 (MN9D/Bcl-2) or neomycin (MN9D/Neo). Overexpression of Bcl-2 in MN9D cells attenuated cell death due to treatment of mitochondrial electron transport inhibitors including N-methyl-4-phenylpyridinium, whereas it did not prevent cell death induced by reagents generating reactive oxygen species including 6-hydroxy-dopamine. Moreover, the rate of glucose uptake in MN9D/Bcl-2 was significantly lower than that in MN9D/Neo after MPP+ treatment. Thus, Bcl-2 may counter aberrations in mitochondrial electron transfer processes by altering energy metabolism within the MN9D cells.
Original language | English |
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Pages (from-to) | 157-167 |
Number of pages | 11 |
Journal | Neurobiology of Disease |
Volume | 2 |
Issue number | 3 |
DOIs | |
State | Published - 1995 |
Keywords
- Bcl-2
- Cell death
- Dopamine
- Glycollate
- MPP
- Sodium pentosan polysulphate