Overexpression of adenovirus-encoded transgenes from the cytomegalovirus immediate early promoter in irradiated tumor cells

D. E.Chu Tang, Richard S. Jennelle, Zhongkai Shi, Robert I. Garver, David P. Carbone, Francisco Loya, Cheng Hui Chang, David T. Curiel

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Efficient expression of therapeutic genes in irradiated tumor cells would facilitate the conversion of a malignant tumor nodule into a cancer vaccine in situ. We reported previously that transgene expression from an adenoviral vector could be markedly enhanced by treating transduced tumor cells with butyrate. In this study, we demonstrated that a similar butyrate effect could be achieved in irradiated tumor cells. In addition, irradiating cells at doses of 2-40 Gy prior to transduction could also amplify recombinant adenoviral transgene products in a cell-type-specific manner. This suggests that adenovirus-mediated gene therapy, radiation therapy, and butyrate-mediated cancer therapy may potentially be formulated into one synergistic protocol for cancer treatment.

Original languageEnglish
Pages (from-to)2117-2124
Number of pages8
JournalHuman Gene Therapy
Volume8
Issue number17
DOIs
StatePublished - Nov 20 1997
Externally publishedYes

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