Out-of-focus signal rejection for in vivo pO2 measurements using two-photon phosphorescence lifetime microscopy

Qi Pian, Baoqiang Li, Ikbal Şencan-Eǧilmez, Xiaojun Cheng, Jay Dubb, Xinyue Huang, Buyin Fu, Srinivasa Rao Allu, Mohammad Abbas Yaseen, Anna Devor, Sergei A. Vinogradov, Sava Sakadžˇić

Research output: Contribution to journalArticlepeer-review

Abstract

Two-photon phosphorescence lifetime microscopy has been a key tool for studying cerebral oxygenation in mice. However, the accuracy of the partial pressure of oxygen (pO2) measurements is affected by out-of-focus signal. In this work, we applied reconfigurable differential aberration imaging to characterize and correct for out-of-focus signal contamination in intravascular pO2 imaging. Our results show that signal contamination is higher in more oxygenated vessels and that it could be effectively removed using the proposed method.

Original languageEnglish
Pages (from-to)159-176
Number of pages18
JournalBiomedical Optics Express
Volume16
Issue number1
DOIs
StatePublished - Jan 2025

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