TY - JOUR
T1 - Out-of-focus signal rejection for in vivo pO2 measurements using two-photon phosphorescence lifetime microscopy
AU - Pian, Qi
AU - Li, Baoqiang
AU - Şencan-Eǧilmez, Ikbal
AU - Cheng, Xiaojun
AU - Dubb, Jay
AU - Huang, Xinyue
AU - Fu, Buyin
AU - Allu, Srinivasa Rao
AU - Yaseen, Mohammad Abbas
AU - Devor, Anna
AU - Vinogradov, Sergei A.
AU - Sakadžˇić, Sava
N1 - Publisher Copyright:
© 2024 Optica Publishing Group.
PY - 2025/1
Y1 - 2025/1
N2 - Two-photon phosphorescence lifetime microscopy has been a key tool for studying cerebral oxygenation in mice. However, the accuracy of the partial pressure of oxygen (pO2) measurements is affected by out-of-focus signal. In this work, we applied reconfigurable differential aberration imaging to characterize and correct for out-of-focus signal contamination in intravascular pO2 imaging. Our results show that signal contamination is higher in more oxygenated vessels and that it could be effectively removed using the proposed method.
AB - Two-photon phosphorescence lifetime microscopy has been a key tool for studying cerebral oxygenation in mice. However, the accuracy of the partial pressure of oxygen (pO2) measurements is affected by out-of-focus signal. In this work, we applied reconfigurable differential aberration imaging to characterize and correct for out-of-focus signal contamination in intravascular pO2 imaging. Our results show that signal contamination is higher in more oxygenated vessels and that it could be effectively removed using the proposed method.
UR - http://www.scopus.com/inward/record.url?scp=85213817171&partnerID=8YFLogxK
U2 - 10.1364/BOE.532084
DO - 10.1364/BOE.532084
M3 - Article
C2 - 39816157
AN - SCOPUS:85213817171
SN - 2156-7085
VL - 16
SP - 159
EP - 176
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
ER -