TY - JOUR
T1 - Osteogenic constituents from Pterospermum acerifolium Willd. flowers
AU - Dixit, Preety
AU - Khan, Mohd Parvez
AU - Swarnkar, Gaurav
AU - Chattopadhyay, N.
AU - Maurya, Rakesh
N1 - Funding Information:
Generous funding from the Ministry of Health and Family Welfare (grant-in-aid) is acknowledged. Preety Dixit and Gaurav Swarnkar are thankful to Council of Scientific and Industrial Research. Mohd Parvez Khan is thankful to Indian Council of Medical Research, New Delhi for the award of Research Fellowship. We are also thankful to SAIF, CDRI for providing spectroscopic data.
PY - 2011/8/1
Y1 - 2011/8/1
N2 - Phytochemical investigation of ethanol extracts of the Pterospermum acerifolium flowers led to the isolation and identification of two new flavones, 4′-(2-methoxy-4-(1,2,3-trihydroxypropyl) phenoxy luteolin (1) and 5,7,3′-trihydroxy-6-O-β-d-glucopyranosyl flavone (2), and one new lactone, 3,5-dihydroxyfuran-2(5H)-one (3) along with 14 known compounds (4-17). The structure of compounds 1-17 was established based on MS, 1D and 2D NMR, spectroscopic analysis. Eight of these compounds (1-6, 8 and 9) were assessed for osteogenic activity by using primary cultures of rat osteoblast. The compounds 1, 3 and 4 significantly stimulated osteoblast differentiation and mineralization as evident from a marked increase in expression of alkaline phosphatase and alizarin red-S staining of osteoblasts.
AB - Phytochemical investigation of ethanol extracts of the Pterospermum acerifolium flowers led to the isolation and identification of two new flavones, 4′-(2-methoxy-4-(1,2,3-trihydroxypropyl) phenoxy luteolin (1) and 5,7,3′-trihydroxy-6-O-β-d-glucopyranosyl flavone (2), and one new lactone, 3,5-dihydroxyfuran-2(5H)-one (3) along with 14 known compounds (4-17). The structure of compounds 1-17 was established based on MS, 1D and 2D NMR, spectroscopic analysis. Eight of these compounds (1-6, 8 and 9) were assessed for osteogenic activity by using primary cultures of rat osteoblast. The compounds 1, 3 and 4 significantly stimulated osteoblast differentiation and mineralization as evident from a marked increase in expression of alkaline phosphatase and alizarin red-S staining of osteoblasts.
KW - Osteoblast differentiation
KW - Pterospermin A
KW - Pterospermin B
KW - Pterospermin C
KW - Pterospermum acerifolium
UR - http://www.scopus.com/inward/record.url?scp=79960379225&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2011.05.087
DO - 10.1016/j.bmcl.2011.05.087
M3 - Article
C2 - 21719287
AN - SCOPUS:79960379225
SN - 0960-894X
VL - 21
SP - 4617
EP - 4621
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 15
ER -