TY - JOUR
T1 - Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology
AU - Krupnick, Alexander S.
AU - Lin, Xue
AU - Li, Wenjun
AU - Okazaki, Mikio
AU - Lai, Jiaming
AU - Sugimoto, Seiichiro
AU - Richardson, Steven B.
AU - Kornfeld, Christopher G.
AU - Garbow, Joel R.
AU - Patterson, Alexander G.
AU - Gelman, Andrew E.
AU - Kreisel, Daniel
N1 - Funding Information:
ACKNOWLEDGMENTS A.S.K. is supported by (i) the American Association for Thoracic Surgery Norman Shumway Research Scholarship; (ii) American Cancer Society Internal Research Grant through the Alvin J Siteman Cancer Center; (iii) Melanoma Research Foundation; and (iv) a grant jointly sponsored by The National Cancer Institute and The Thoracic Surgery Foundation for Research and Education (1K08CA131097). D.K. is supported by a grant jointly sponsored by The National Heart, Lung and Blood Institute and The Thoracic Surgery Foundation for Research and Education (1K08HL083983-01). We thank Arlene Aligori for the medical illustrations.
PY - 2009
Y1 - 2009
N2 - Unlike transplantation of other solid organs, vascularized mouse lung transplantation has only recently been developed. In this protocol, we describe a detailed method for performing a vascularized and aerated mouse orthotopic lung transplant, which to date represents the most physiological mouse model of lung transplantation. The procedure is divided into two separate portions consisting of donor harvest followed by implantation using the cuff technique for bronchovascular anastomoses. After a training period spanning several months, the procedure can be successfully mastered and, in experienced hands, requires ∼90 min to perform. After an initial learning curve, perioperative survival is close to 100%. As the donor hematopoietic cells in the transplanted lung are replaced by those of the host over time, thereby creating a 'chimeric lung,' this model represents a novel research tool for the study of transplantation biology as well as other disease processes, such as malignancies.
AB - Unlike transplantation of other solid organs, vascularized mouse lung transplantation has only recently been developed. In this protocol, we describe a detailed method for performing a vascularized and aerated mouse orthotopic lung transplant, which to date represents the most physiological mouse model of lung transplantation. The procedure is divided into two separate portions consisting of donor harvest followed by implantation using the cuff technique for bronchovascular anastomoses. After a training period spanning several months, the procedure can be successfully mastered and, in experienced hands, requires ∼90 min to perform. After an initial learning curve, perioperative survival is close to 100%. As the donor hematopoietic cells in the transplanted lung are replaced by those of the host over time, thereby creating a 'chimeric lung,' this model represents a novel research tool for the study of transplantation biology as well as other disease processes, such as malignancies.
UR - http://www.scopus.com/inward/record.url?scp=61449095643&partnerID=8YFLogxK
U2 - 10.1038/nprot.2008.218
DO - 10.1038/nprot.2008.218
M3 - Article
C2 - 19131960
AN - SCOPUS:61449095643
SN - 1754-2189
VL - 4
SP - 86
EP - 93
JO - Nature Protocols
JF - Nature Protocols
IS - 1
ER -