Orsay, santeuil and le blanc viruses primarily infect intestinal cells in caenorhabditis nematodes

Carl J. Franz, Hilary Renshaw, Lise Frezal, Yanfang Jiang, Marie Anne Félix, David Wang

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


The discoveries of Orsay, Santeuil and Le Blanc viruses, three viruses infecting either Caenorhabditis elegans or its relative Caenorhabditis briggsae, enable the study of virus-host interactions using natural pathogens of these two well-established model organisms. We characterized the tissue tropism of infection in Caenorhabditis nematodes by these viruses. Using immunofluorescence assays targeting proteins from each of the viruses, and in situ hybridization, we demonstrate viral proteins and RNAs localize to intestinal cells in larval stage Caenorhabditis nematodes. Viral proteins were detected in one to six of the 20 intestinal cells present in Caenorhabditis nematodes. In Orsay virus-infected C. elegans, viral proteins were detected as early as 6. h post-infection. The RNA-dependent RNA polymerase and capsid proteins of Orsay virus exhibited different subcellular localization patterns. Collectively, these observations provide the first experimental insights into viral protein expression in any nematode host, and broaden our understanding of viral infection in Caenorhabditis nematodes.

Original languageEnglish
Pages (from-to)255-264
Number of pages10
StatePublished - Jan 5 2014


  • Immunofluorescence
  • Nematode virology
  • Tissue tropism


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