Origins and formation of histone methylation across the human cell cycle

Barry M. Zee, Laura Mae P. Britton, Daniel Wolle, Devorah M. Haberman, Benjamin A. Garcia

Research output: Contribution to journalArticlepeer-review

69 Scopus citations


The connections between various nuclear processes and specific histone posttranslational modifications are dependent to a large extent on the acquisition of those modifications after histone synthesis. The reestablishment of histone posttranslational modifications after S phase is especially critical for H3K9 and H3K27 trimethylation, both of which are linked with epigenetic memory and must be stably transmitted from one cellular generation to the next. This report uses a proteomic strategy to interrogate how and when the cell coordinates the formation of histone posttranslational modifications during division. Paramount among the findings is that H3K9 and H3K27 trimethylation begins during S phase but is completed only during the subsequent G1 phase via two distinct pathways from the unmodified and preexisting dimethylated states. In short, we have systematically characterized the temporal origins and methylation pathways for histone posttranslational modifications during the cell cycle.

Original languageEnglish
Pages (from-to)2503-2514
Number of pages12
JournalMolecular and cellular biology
Issue number13
StatePublished - Jul 2012


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