TY - GEN
T1 - Optogenetic control of cytokinesis shows that polarized trafficking governs the process
AU - Castillo-Badillo, Jean A.
AU - Meshik, Xenia
AU - Harlalka, Suyash
AU - Gautam, N.
N1 - Funding Information:
This work was funded by the NIH through NIGMS grants GM069027, GM107370, and GM122577. We thank Vani Kalyanaraman for DNA constructs and Patrick O’Neill for helpful discussions.
Publisher Copyright:
© 2019 SPIE.
PY - 2019
Y1 - 2019
N2 - Optogenetics allows for the study of signaling pathways during a variety of cellular processes. Here we use subcellular optogenetics to study the role of vesicular trafficking in cytokinesis. Optogenetically activating RhoA in the cell midline induces cytokinesis and intercellular bridge formation. RhoA activates the actomyosin contractility network, leading to retrograde plasma membrane flow, localized decrease in membrane tension, and increase in endocytosis at the cell middle. Endocytic vesicles and trafficking proteins localize to the intercellular bridge. Perturbation of these proteins inhibits furrow formation. Thus polarized endocytosis and exocytosis in the first steps of cytokinesis provide new membrane to the middle of the cell and allow the cell to build the cleavage furrow and intercellular bridge.
AB - Optogenetics allows for the study of signaling pathways during a variety of cellular processes. Here we use subcellular optogenetics to study the role of vesicular trafficking in cytokinesis. Optogenetically activating RhoA in the cell midline induces cytokinesis and intercellular bridge formation. RhoA activates the actomyosin contractility network, leading to retrograde plasma membrane flow, localized decrease in membrane tension, and increase in endocytosis at the cell middle. Endocytic vesicles and trafficking proteins localize to the intercellular bridge. Perturbation of these proteins inhibits furrow formation. Thus polarized endocytosis and exocytosis in the first steps of cytokinesis provide new membrane to the middle of the cell and allow the cell to build the cleavage furrow and intercellular bridge.
UR - http://www.scopus.com/inward/record.url?scp=85064403121&partnerID=8YFLogxK
U2 - 10.1117/12.2508792
DO - 10.1117/12.2508792
M3 - Conference contribution
AN - SCOPUS:85064403121
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optogenetics and Optical Manipulation 2019
A2 - Jansen, E. Duco
A2 - Mohanty, Samarendra K.
PB - SPIE
T2 - Optogenetics and Optical Manipulation 2019
Y2 - 2 February 2019 through 3 February 2019
ER -