@article{bc881fa832634b75b4a6bdcfcb1ea169,
title = "Optimal cytokine stimulation for the enhanced generation of leukemic dendritic cells in short-term culture",
abstract = "Dendritic cells (DCs) are professional antigen presenting cells derived from myeloid or lymphoid precursors. Functional DCs have been generated from the malignant counterpart of these precursor cells. Herein, we describe the generation of DCs from different leukemias and determine the optimal culture conditions with minimal manipulation. Primary leukemic cells were cultured for 1, 3, and 5 days in 11 different cytokine combinations and analyzed for the expression of a mature DC phenotype. Optimal growth and DC characteristics were obtained with GM-CSF, FL, and SCF in 3-5 day cultures, suggesting a practical strategy for the immunotherapy of leukemia.",
keywords = "AML, Culture, Cytokine, Dendritic cell, Immunotherapy, Leukemia",
author = "Nicki Panoskaltsis and Belanger, {Todd J.} and Liesveld, {Jane L.} and Abboud, {Camille N.}",
note = "Funding Information: This research was supported by the American Cancer Society, grant DHP-173, and partially by an Amgen Fellowship for N.P. Jane Liesveld is a Leukemia Society of America Clinical Scholar. The authors thank Dr Athanasios Mantalaris for his critical review of the manuscript as well as Abbe Harbol and Karen Rosell for their expert technical assistance. We also thank the patients who donated blood and bone marrow for the completion of these studies, and to the nurses, nurse practitioners, residents, fellows, and attending hematologists at The University of Rochester Medical Center, Strong Memorial Hospital who kindly collected the samples. N. Panoskaltsis procured the samples, performed the cell cultures and FACS staining, mixed leukocyte reactions, wrote the manuscript, and provided the critical revision for the paper. T.J. Belanger provided technical support. J.L. Liesveld and C.N. Abboud contributed to the manuscript preparation and revision, obtained funding, and gave final approval.",
year = "2002",
doi = "10.1016/S0145-2126(01)00104-7",
language = "English",
volume = "26",
pages = "191--201",
journal = "Leukemia Research",
issn = "0145-2126",
number = "2",
}