Optimal cytokine stimulation for the enhanced generation of leukemic dendritic cells in short-term culture

Nicki Panoskaltsis, Todd J. Belanger, Jane L. Liesveld, Camille N. Abboud

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Dendritic cells (DCs) are professional antigen presenting cells derived from myeloid or lymphoid precursors. Functional DCs have been generated from the malignant counterpart of these precursor cells. Herein, we describe the generation of DCs from different leukemias and determine the optimal culture conditions with minimal manipulation. Primary leukemic cells were cultured for 1, 3, and 5 days in 11 different cytokine combinations and analyzed for the expression of a mature DC phenotype. Optimal growth and DC characteristics were obtained with GM-CSF, FL, and SCF in 3-5 day cultures, suggesting a practical strategy for the immunotherapy of leukemia.

Original languageEnglish
Pages (from-to)191-201
Number of pages11
JournalLeukemia Research
Volume26
Issue number2
DOIs
StatePublished - Jan 15 2002
Externally publishedYes

Keywords

  • AML
  • Culture
  • Cytokine
  • Dendritic cell
  • Immunotherapy
  • Leukemia

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