Optical-resolution photoacoustic microscopy of amyloid-β deposits in vivo

Song Hu, Ping Yan, Konstantin Maslov, Jin Moo Lee, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations


Advances in high-resolution imaging have permitted microscopic observations within the brains of living animals. Applied to Alzheimer's disease (AD) mouse models, multiphoton microscopy has opened a new window to study the real-time appearance and growth of amyloid plaques. Here, we report an alternative technology-optical-resolution photoacoustic microscopy (OR-PAM)-for in vivo imaging of amyloid plaques in a transgenic AD mouse model. In vivo validation using multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window. In vivo transcranial OR-PAM imaging of amyloid plaques is highly likely once the imaging parameters are optimized.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2010
StatePublished - 2010
EventPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2010
Country/TerritoryUnited States
CitySan Francisco, CA


  • Alzheimer's disease
  • Amyloid-β
  • Dual contrast
  • Optical-resolution photoacoustic microscopy


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