Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene

Soo Young Park, Xiaoyu Wang, Zhongyi Chen, Alvin C. Powers, Mark A. Magnuson, W. Steven Head, David W. Piston, Graeme I. Bell

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

We generated transgenic mice expressing firefly (Photinus pyralis) luciferase (luc) under the control of the mouse insulin 1 promoter (MIP). The mice have normal glucose tolerance and pancreatic islet architecture. The luciferase-expressing beta cells can be readily visualized in living mice using whole-body bioluminescent imaging. The MIP-luc transgenic mice may be useful for monitoring changes in beta cell function or mass in living animals with normal or altered metabolic states.

Original languageEnglish
Pages (from-to)80-86
Number of pages7
JournalGenesis
Volume43
Issue number2
DOIs
StatePublished - Oct 2005

Keywords

  • Beta cell
  • Firefly luciferase
  • Imaging
  • Insulin
  • Transgenic

Fingerprint Dive into the research topics of 'Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene'. Together they form a unique fingerprint.

Cite this