Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene

Soo Young Park; Xiaoyu Wang; Zhongyi Chen; Alvin C. Powers; Mark A. Magnuson; W. Steven Head; David W. Piston; Graeme I. Bell

doi:10.1002/gene.20157

Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene

Soo Young Park, Xiaoyu Wang, Zhongyi Chen, Alvin C. Powers, Mark A. Magnuson, W. Steven Head, David W. Piston, Graeme I. Bell

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

We generated transgenic mice expressing firefly (Photinus pyralis) luciferase (luc) under the control of the mouse insulin 1 promoter (MIP). The mice have normal glucose tolerance and pancreatic islet architecture. The luciferase-expressing beta cells can be readily visualized in living mice using whole-body bioluminescent imaging. The MIP-luc transgenic mice may be useful for monitoring changes in beta cell function or mass in living animals with normal or altered metabolic states.

Original language	English
Pages (from-to)	80-86
Number of pages	7
Journal	Genesis
Volume	43
Issue number	2
DOIs	https://doi.org/10.1002/gene.20157
State	Published - Oct 2005

Keywords

Beta cell
Firefly luciferase
Imaging
Insulin
Transgenic

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10.1002/gene.20157

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title = "Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene",

abstract = "We generated transgenic mice expressing firefly (Photinus pyralis) luciferase (luc) under the control of the mouse insulin 1 promoter (MIP). The mice have normal glucose tolerance and pancreatic islet architecture. The luciferase-expressing beta cells can be readily visualized in living mice using whole-body bioluminescent imaging. The MIP-luc transgenic mice may be useful for monitoring changes in beta cell function or mass in living animals with normal or altered metabolic states.",

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AU - Park, Soo Young

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AU - Chen, Zhongyi

AU - Powers, Alvin C.

AU - Magnuson, Mark A.

AU - Head, W. Steven

AU - Piston, David W.

AU - Bell, Graeme I.

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AB - We generated transgenic mice expressing firefly (Photinus pyralis) luciferase (luc) under the control of the mouse insulin 1 promoter (MIP). The mice have normal glucose tolerance and pancreatic islet architecture. The luciferase-expressing beta cells can be readily visualized in living mice using whole-body bioluminescent imaging. The MIP-luc transgenic mice may be useful for monitoring changes in beta cell function or mass in living animals with normal or altered metabolic states.

KW - Beta cell

KW - Firefly luciferase

KW - Imaging

KW - Insulin

KW - Transgenic

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Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene

Abstract

Keywords

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