Optical dysfunction of the crystalline lens in aquaporin-0-deficient mice

Alan Shiels, Steven Bassnett, Kulandaiappa Varadaraj, Richard Mathias, Kristin Al-Ghoul, Jer Kuszak, Dorit Donoviel, Stan Lilleberg, Glenn Friedrich, Brian Zambrowicz

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

Aquaporin-0 (AQP0), a water transport channel protein, is the major intrinsic protein (MIP) of lens fiber cell plasma membranes. Mice deficient in the gene for AQP0 (Aqp0, Mip) were generated from a library of gene trap embryo stem cells. Sequence analysis showed that the gene trap vector had inserted into the first exon of Aqp0, causing a null mutation as verified by RNA blotting and immunochemistry. At 3 wk of age (postnatal day 21), lenses from null mice (Aqp0-/-) contained polymorphic opacities, whereas lenses from heterozygous mice (Aqp0+/-) were transparent and did not develop frank opacities until ∼24 wk of age. Osmotic water permeability values for Aqp0+/- and Aqp0-/- lenses were reduced to ∼46% and ∼20% of wild-type values, respectively, and the focusing power of Aqp0+/- lenses was significantly lower than that of wild type. These findings show that heterozygous loss of AQPO is sufficient to trigger cataractogenesis in mice and suggest that this MIP is required for optimal focusing of the crystalline lens.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalPhysiological genomics
Volume2002
Issue number7
StatePublished - Jan 2002

Keywords

  • Cataract
  • Gene trap
  • Mouse
  • Water channel

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