TY - JOUR
T1 - Ontogenic and phylogenic studies of intestinal, hepatic, and placental alkaline phosphatases. Evidence that intestinal alkaline phosphatase is a late evolutionary development
AU - Komoda, T.
AU - Koyama, I.
AU - Nagata, A.
AU - Sakagishi, Y.
AU - DeSchryver-Kecskemeti, K.
AU - Alpers, David H.
N1 - Funding Information:
Received August 2, 1985. Accepted January 17, 1986. Address requests for reprints to: Dr. David H. Alpers, Gastrointestinal Division, Department of Medicine, Washington University School of Medicine, 9934 Clinical Sciences Building, Box 8124, 66p South Euclid, St. Louis, Missouri 63110. This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan, by the Exchange Visitor Program No. P-1-153 from Washington University, and by National Institutes of Health grant AM14038. 0 1986 by the American Gastroenterological 0016.5085/86/$3.50
PY - 1986/8
Y1 - 1986/8
N2 - We have studied the intrauterine development of guinea pig and rat organ phosphatases using biochemical, immunologic, and histologic techniques. In all organs tested the features of the adult phosphatase activity were achieved during the second or third gestational phases. In the rat, the tissue-unspecific phosphatase activity was found by immunoprecipitation with antiserum to rat liver phosphatase in all gestational phases in liver and placenta. The high liver activity in the first phase of gestation corresponded to hematopoietic cells. Hepatocyte phosphatase did not appear until the second gestational phase. The tissue-unspecific phosphatase activity was found in the first and second gestational phase in surface intestinal epithelial cells, even after crypt formation occurred. Once phosphatase appeared in enterocytes on villi, only the intestinal-type enzyme was detected. Alkaline phosphatase was measured in the liver and intestine from animals in various phyla. In fish and reptiles, the intestinal activity had the enzymatic characteristics of the tissue-unspecific enzyme. The appearance of intestinal alkaline phosphatase with unique properties and high specific activity is a characteristic of mammals.
AB - We have studied the intrauterine development of guinea pig and rat organ phosphatases using biochemical, immunologic, and histologic techniques. In all organs tested the features of the adult phosphatase activity were achieved during the second or third gestational phases. In the rat, the tissue-unspecific phosphatase activity was found by immunoprecipitation with antiserum to rat liver phosphatase in all gestational phases in liver and placenta. The high liver activity in the first phase of gestation corresponded to hematopoietic cells. Hepatocyte phosphatase did not appear until the second gestational phase. The tissue-unspecific phosphatase activity was found in the first and second gestational phase in surface intestinal epithelial cells, even after crypt formation occurred. Once phosphatase appeared in enterocytes on villi, only the intestinal-type enzyme was detected. Alkaline phosphatase was measured in the liver and intestine from animals in various phyla. In fish and reptiles, the intestinal activity had the enzymatic characteristics of the tissue-unspecific enzyme. The appearance of intestinal alkaline phosphatase with unique properties and high specific activity is a characteristic of mammals.
UR - http://www.scopus.com/inward/record.url?scp=0022641144&partnerID=8YFLogxK
U2 - 10.1016/0016-5085(86)90558-5
DO - 10.1016/0016-5085(86)90558-5
M3 - Article
C2 - 3522339
AN - SCOPUS:0022641144
VL - 91
SP - 277
EP - 286
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 2
ER -