TY - JOUR
T1 - Online identification of potential antioxidant components and evaluation of DNA oxidative damage protection ability in Prunus persica flowers
AU - Sun, Mimi
AU - Lei, Xinyu
AU - Lan, Xin
AU - Lin, Zongtao
AU - Xu, Hongbo
AU - Chen, Shizhong
N1 - Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2024/12/1
Y1 - 2024/12/1
N2 - A high performance liquid chromatography-ultraviolet-visible detector-electrospray ionization-ion trap-time-of-flight-mass spectrometry-total antioxidant capacity determination (HPLC-UVD-ESI-IT-TOF-MS-TACD) new online technique was developed for efficient screening of potential antioxidant active components in Prunus persica flowers (PPF) from 4 origins. Through this online system, 46 compounds were initially identified, while 20 compounds with DPPH binding activity and 21 compounds with FRAP binding activity were detected. The antioxidant activities of 9 compounds obtained from the screening were then validated in DNA oxidative damage protection study. The results showed that this online system can cope well with the complexity of the samples. This also provides technical basis for rapid screening of antioxidant resources of PPF. In short, this study made the chemical composition of PPF more abundant and its potential antioxidant active compounds more explicit, which provided new ideas for the detection and development of natural antioxidants and provided scientific basis for PPF as functional food.
AB - A high performance liquid chromatography-ultraviolet-visible detector-electrospray ionization-ion trap-time-of-flight-mass spectrometry-total antioxidant capacity determination (HPLC-UVD-ESI-IT-TOF-MS-TACD) new online technique was developed for efficient screening of potential antioxidant active components in Prunus persica flowers (PPF) from 4 origins. Through this online system, 46 compounds were initially identified, while 20 compounds with DPPH binding activity and 21 compounds with FRAP binding activity were detected. The antioxidant activities of 9 compounds obtained from the screening were then validated in DNA oxidative damage protection study. The results showed that this online system can cope well with the complexity of the samples. This also provides technical basis for rapid screening of antioxidant resources of PPF. In short, this study made the chemical composition of PPF more abundant and its potential antioxidant active compounds more explicit, which provided new ideas for the detection and development of natural antioxidants and provided scientific basis for PPF as functional food.
KW - DNA oxidative damage
KW - HPLC-UVD-ESI-IT-TOF-MS-TACD
KW - Natural antioxidants
KW - Prunus persica flowers
UR - http://www.scopus.com/inward/record.url?scp=85201784238&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2024.126702
DO - 10.1016/j.talanta.2024.126702
M3 - Article
C2 - 39180873
AN - SCOPUS:85201784238
SN - 0039-9140
VL - 280
JO - Talanta
JF - Talanta
M1 - 126702
ER -