Oncomodulin is abundant in the organ of Corti

Michael T. Henzl, Osamu Shibasaki, Thomas H. Comegys, Isolde Thalmann, Ruediger Thalmann

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31 Scopus citations

Abstract

A small, acidic Ca2+-binding protein (CBP-15) was recently detected in extracts of the mammalian auditory receptor organ, the organ of Corti [Senarita et al. (1995) Hear. Res. 90, 169-175]. N-terminal sequence data for CBP-15 [Thalmann et al. (1995) Biochem. Biophys. Res. Commun. 215, 142-147] implied membership in the parvalbumin family and possible identity with the mammalian β-parvalbumin oncomodulin. As shown herein, the latter conclusion is supported by strong cross-reactivity between CBP-15 and isoform-specific antibodies to oncomodulin. Moreover, we have succeeded in amplifying the guinea pig CBP-15 coding sequence from organ of Corti cDNA using degenerate oligonucleotide primers based on the rat oncomodulin sequence. The deduced amino acid sequence of guinea pig CBP-15 displays 90%, 92%, and 98% identity with mouse, rat, and human oncomodulin isoforms. Demonstration of the presence of oncomodulin in the organ of Corti is the first documentation of this substance in a postnatal mammalian tissue.

Original languageEnglish
Pages (from-to)105-111
Number of pages7
JournalHearing research
Volume106
Issue number1-2
DOIs
StatePublished - Apr 1997

Keywords

  • guinea pig
  • oncomodulin
  • organ of Corti
  • parvalbumin

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