TY - JOUR
T1 - Oncogenic potential of the DNA replication licensing protein CDT1
AU - Arentson, Elizabeth
AU - Faloon, Patrick
AU - Seo, Junghee
AU - Moon, Eunpyo
AU - Studts, Joey M.
AU - Fremont, Daved H.
AU - Choi, Kyunghee
N1 - Funding Information:
We thank Douglas Dean, Steven Dowdy, and Linda Van Dyk for helpful discussion with cell cycle analyses. We thank Kathy Sheehan for help with the antibody generation, Hyung Kyoo Kang with the help of Western blot analyses, Paul Allen and Barry Sleckman for rag?2?/? mice, and Darren Kraemelmeyer for animal husbandry. We would like to thank Rainer Brachmann, Yun Shin Chung, Marco Colonna, Douglas Dean, Igor Roninson, Robert Schreiber and Wen Jie Zhang for helpful comments on the manuscript.
PY - 2002/2/14
Y1 - 2002/2/14
N2 - The expression of a gene, designated as Retroviral insertion site (Ris)2, was activated by retroviral DNA integration in an immortalized primitive erythroid cell line, EB-PE. Ris2 was also expressed at high levels in all human tumor cell lines analysed. Consistently, NIH3T3 fibroblasts overexpressing Ris2 formed tumors in Rag2-/- mice when injected subcutaneously. The putative RIS2 protein shows a high sequence similarity to Xenopus CDT1, Drosophila DUP, and human CDT1, a newly identified DNA replication licensing protein, suggesting that Ris2 is a mouse homologue of CDT1. Cells overexpressing Ris2/Cdt1 exhibited a quicker entry into S phase when released from serum starvation compared to controls. Our results suggest that CDT1, an essential licensing protein for DNA replication, can function as an oncogene in mammals.
AB - The expression of a gene, designated as Retroviral insertion site (Ris)2, was activated by retroviral DNA integration in an immortalized primitive erythroid cell line, EB-PE. Ris2 was also expressed at high levels in all human tumor cell lines analysed. Consistently, NIH3T3 fibroblasts overexpressing Ris2 formed tumors in Rag2-/- mice when injected subcutaneously. The putative RIS2 protein shows a high sequence similarity to Xenopus CDT1, Drosophila DUP, and human CDT1, a newly identified DNA replication licensing protein, suggesting that Ris2 is a mouse homologue of CDT1. Cells overexpressing Ris2/Cdt1 exhibited a quicker entry into S phase when released from serum starvation compared to controls. Our results suggest that CDT1, an essential licensing protein for DNA replication, can function as an oncogene in mammals.
KW - CDT1
KW - Oncogene
KW - Retroviral insertional activation
UR - https://www.scopus.com/pages/publications/0037075195
U2 - 10.1038/sj/onc/1205175
DO - 10.1038/sj/onc/1205175
M3 - Article
C2 - 11850834
AN - SCOPUS:0037075195
SN - 0950-9232
VL - 21
SP - 1150
EP - 1158
JO - Oncogene
JF - Oncogene
IS - 8
ER -