Odontoblast TRPC5 channels signal cold pain in teeth

Laura Bernal; Pamela Sotelo-Hitschfeld; Christine König; Viktor Sinica; Amanda Wyatt; Zoltan Winter; Alexander Hein; Filip Touska; Susanne Reinhardt; Aaron Tragl; Ricardo Kusuda; Philipp Wartenberg; Allen Sclaroff; John D. Pfeifer; Fabien Ectors; Andreas Dahl; Marc Freichel; Viktorie Vlachova; Sebastian Brauchi; Carolina Roza; Ulrich Boehm; David E. Clapham; Jochen K. Lennerz; Katharina Zimmermann

doi:10.1126/sciadv.abf5567

Odontoblast TRPC5 channels signal cold pain in teeth

Laura Bernal, Pamela Sotelo-Hitschfeld, Christine König, Viktor Sinica, Amanda Wyatt, Zoltan Winter, Alexander Hein, Filip Touska, Susanne Reinhardt, Aaron Tragl, Ricardo Kusuda, Philipp Wartenberg, Allen Sclaroff, John D. Pfeifer, Fabien Ectors, Andreas Dahl, Marc Freichel, Viktorie Vlachova, Sebastian Brauchi, Carolina RozaUlrich Boehm, David E. Clapham, Jochen K. Lennerz, Katharina Zimmermann

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Abstract

Teeth are composed of many tissues, covered by an inflexible and obdurate enamel. Unlike most other tissues, teeth become extremely cold sensitive when inflamed. The mechanisms of this cold sensation are not understood. Here, we clarify the molecular and cellular components of the dental cold sensing system and show that sensory transduction of cold stimuli in teeth requires odontoblasts. TRPC5 is a cold sensor in healthy teeth and, with TRPA1, is sufficient for cold sensing. The odontoblast appears as the direct site of TRPC5 cold transduction and provides a mechanism for prolonged cold sensing via TRPC5’s relative sensitivity to intracellular calcium and lack of desensitization. Our data provide concrete functional evidence that equipping odontoblasts with the cold-sensor TRPC5 expands traditional odontoblast functions and renders it a previously unknown integral cellular component of the dental cold sensing system.

Original language	English
Article number	eabf5567
Journal	Science Advances
Volume	7
Issue number	13
DOIs	https://doi.org/10.1126/sciadv.abf5567
State	Published - Mar 2021

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Bernal, L., Sotelo-Hitschfeld, P., König, C., Sinica, V., Wyatt, A., Winter, Z., Hein, A., Touska, F., Reinhardt, S., Tragl, A., Kusuda, R., Wartenberg, P., Sclaroff, A., Pfeifer, J. D., Ectors, F., Dahl, A., Freichel, M., Vlachova, V., Brauchi, S., ... Zimmermann, K. (2021). Odontoblast TRPC5 channels signal cold pain in teeth. Science Advances, 7(13), Article eabf5567. https://doi.org/10.1126/sciadv.abf5567

@article{cb10f7738663424193484e37734062f9,

title = "Odontoblast TRPC5 channels signal cold pain in teeth",

abstract = "Teeth are composed of many tissues, covered by an inflexible and obdurate enamel. Unlike most other tissues, teeth become extremely cold sensitive when inflamed. The mechanisms of this cold sensation are not understood. Here, we clarify the molecular and cellular components of the dental cold sensing system and show that sensory transduction of cold stimuli in teeth requires odontoblasts. TRPC5 is a cold sensor in healthy teeth and, with TRPA1, is sufficient for cold sensing. The odontoblast appears as the direct site of TRPC5 cold transduction and provides a mechanism for prolonged cold sensing via TRPC5{\textquoteright}s relative sensitivity to intracellular calcium and lack of desensitization. Our data provide concrete functional evidence that equipping odontoblasts with the cold-sensor TRPC5 expands traditional odontoblast functions and renders it a previously unknown integral cellular component of the dental cold sensing system.",

author = "Laura Bernal and Pamela Sotelo-Hitschfeld and Christine K{\"o}nig and Viktor Sinica and Amanda Wyatt and Zoltan Winter and Alexander Hein and Filip Touska and Susanne Reinhardt and Aaron Tragl and Ricardo Kusuda and Philipp Wartenberg and Allen Sclaroff and Pfeifer, {John D.} and Fabien Ectors and Andreas Dahl and Marc Freichel and Viktorie Vlachova and Sebastian Brauchi and Carolina Roza and Ulrich Boehm and Clapham, {David E.} and Lennerz, {Jochen K.} and Katharina Zimmermann",

note = "Publisher Copyright: Copyright {\textcopyright} 2021 The Authors, some rights reserved.",

year = "2021",

month = mar,

doi = "10.1126/sciadv.abf5567",

language = "English",

volume = "7",

journal = "Science Advances",

issn = "2375-2548",

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Bernal, L, Sotelo-Hitschfeld, P, König, C, Sinica, V, Wyatt, A, Winter, Z, Hein, A, Touska, F, Reinhardt, S, Tragl, A, Kusuda, R, Wartenberg, P, Sclaroff, A, Pfeifer, JD, Ectors, F, Dahl, A, Freichel, M, Vlachova, V, Brauchi, S, Roza, C, Boehm, U, Clapham, DE, Lennerz, JK & Zimmermann, K 2021, 'Odontoblast TRPC5 channels signal cold pain in teeth', Science Advances, vol. 7, no. 13, eabf5567. https://doi.org/10.1126/sciadv.abf5567

TY - JOUR

T1 - Odontoblast TRPC5 channels signal cold pain in teeth

AU - Bernal, Laura

AU - Sotelo-Hitschfeld, Pamela

AU - König, Christine

AU - Sinica, Viktor

AU - Wyatt, Amanda

AU - Winter, Zoltan

AU - Hein, Alexander

AU - Touska, Filip

AU - Reinhardt, Susanne

AU - Tragl, Aaron

AU - Kusuda, Ricardo

AU - Wartenberg, Philipp

AU - Sclaroff, Allen

AU - Pfeifer, John D.

AU - Ectors, Fabien

AU - Dahl, Andreas

AU - Freichel, Marc

AU - Vlachova, Viktorie

AU - Brauchi, Sebastian

AU - Roza, Carolina

AU - Boehm, Ulrich

AU - Clapham, David E.

AU - Lennerz, Jochen K.

AU - Zimmermann, Katharina

PY - 2021/3

Y1 - 2021/3

N2 - Teeth are composed of many tissues, covered by an inflexible and obdurate enamel. Unlike most other tissues, teeth become extremely cold sensitive when inflamed. The mechanisms of this cold sensation are not understood. Here, we clarify the molecular and cellular components of the dental cold sensing system and show that sensory transduction of cold stimuli in teeth requires odontoblasts. TRPC5 is a cold sensor in healthy teeth and, with TRPA1, is sufficient for cold sensing. The odontoblast appears as the direct site of TRPC5 cold transduction and provides a mechanism for prolonged cold sensing via TRPC5’s relative sensitivity to intracellular calcium and lack of desensitization. Our data provide concrete functional evidence that equipping odontoblasts with the cold-sensor TRPC5 expands traditional odontoblast functions and renders it a previously unknown integral cellular component of the dental cold sensing system.

AB - Teeth are composed of many tissues, covered by an inflexible and obdurate enamel. Unlike most other tissues, teeth become extremely cold sensitive when inflamed. The mechanisms of this cold sensation are not understood. Here, we clarify the molecular and cellular components of the dental cold sensing system and show that sensory transduction of cold stimuli in teeth requires odontoblasts. TRPC5 is a cold sensor in healthy teeth and, with TRPA1, is sufficient for cold sensing. The odontoblast appears as the direct site of TRPC5 cold transduction and provides a mechanism for prolonged cold sensing via TRPC5’s relative sensitivity to intracellular calcium and lack of desensitization. Our data provide concrete functional evidence that equipping odontoblasts with the cold-sensor TRPC5 expands traditional odontoblast functions and renders it a previously unknown integral cellular component of the dental cold sensing system.

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U2 - 10.1126/sciadv.abf5567

DO - 10.1126/sciadv.abf5567

M3 - Article

C2 - 33771873

AN - SCOPUS:85103532321

SN - 2375-2548

VL - 7

JO - Science Advances

JF - Science Advances

IS - 13

M1 - eabf5567

ER -

Odontoblast TRPC5 channels signal cold pain in teeth

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