TY - JOUR
T1 - O-GlcNAc peptide epoxyketones are recognized by mammalian proteasomes
AU - Witte, Martin D.
AU - Florea, Bogdan I.
AU - Verdoes, Martijn
AU - Adeyanju, Oloruntosin
AU - Van Der Marel, Gijs A.
AU - Overkleeft, Herman S.
PY - 2009/9/2
Y1 - 2009/9/2
N2 - (Chemical Equation Presented) Cytosolic and nuclear proteins may be subject to both O-GlcNAcylation and proteasomal degradation. By means of activity-based profiling, we demonstrate that O-GlcNAc serinecontaining peptide epoxyketones bind to the proteasome catalytic active sites and thus provide the first clear evidence that proteasomes recognize peptides post-translationally modified with a GlcNAc moiety.
AB - (Chemical Equation Presented) Cytosolic and nuclear proteins may be subject to both O-GlcNAcylation and proteasomal degradation. By means of activity-based profiling, we demonstrate that O-GlcNAc serinecontaining peptide epoxyketones bind to the proteasome catalytic active sites and thus provide the first clear evidence that proteasomes recognize peptides post-translationally modified with a GlcNAc moiety.
UR - http://www.scopus.com/inward/record.url?scp=69349102023&partnerID=8YFLogxK
U2 - 10.1021/ja901231w
DO - 10.1021/ja901231w
M3 - Article
C2 - 19658393
AN - SCOPUS:69349102023
VL - 131
SP - 12064
EP - 12065
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 34
ER -