TY - JOUR
T1 - Nutrient-enhanced diet reduces noise-induced damage to the inner ear and hearing loss
AU - Le Prell, Colleen G.
AU - Gagnon, Patricia M.
AU - Bennett, David C.
AU - Ohlemiller, Kevin K.
N1 - Funding Information:
Portions of this research were presented at the 32nd Midwinter Meeting of the Association for Research in Otolaryngology. 138 The project described was supported by SBIR 1 R43 DC008710 from the National Institute On Deafness And Other Communication Disorders. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute On Deafness And Other Communication Disorders or the National Institutes of Health. This project was awarded to OtoMedicine, Inc., with subcontracts awarded to Washington University (KKO), and the University of Florida (CGL). The College of Public Health and Health Professions at the University of Florida requires the following statement for all activities with direct support from industry; “Acceptance of support does not constitute endorsement of this sponsor by the College of Public Health and Health Professions, its departments, or members of the College.” The authors thank Josef Miller and Peter Boxer for comments on earlier versions of this manuscript, and Marissa Rosa and Kari Morgenstein for editorial assistance. We also thank Mandy Dossat at the University of Florida and David Robbins at Harlan-Teklad for technical assistance.
Funding Information:
Supported by Grant SBIR 1 R43 DC008710 from the National Institute on Deafness and Other Communication Disorders and by an award from OtoMedicine, Inc. , with subcontracts to Washington University (to K.K.O.) and the University of Florida (to C.G.L.).
PY - 2011/7
Y1 - 2011/7
N2 - Oxidative stress has been implicated broadly as a cause of cell death and neural degeneration in multiple disease conditions; however, the evidence for successful intervention with dietary antioxidant manipulations has been mixed. In this study, we investigated the potential for protection of cells in the inner ear using a dietary supplement with multiple antioxidant components, which were selected for their potential interactive effectiveness. Protection against permanent threshold shift (PTS) was observed in CBA/J mice maintained on a diet supplemented with a combination of β-carotene, vitamins C and E, and magnesium when compared with PTS in control mice maintained on a nutritionally complete control diet. Although hair cell survival was not enhanced, noise-induced loss of type II fibrocytes in the lateral wall was significantly reduced (P < 0.05), and there was a trend toward less noise-induced loss in strial cell density in animals maintained on the supplemented diet. Taken together, our data suggest that prenoise oral treatment with the high-nutrient diet can protect cells in the inner ear and reduce PTS in mice. The demonstration of functional and morphologic preservation of cells in the inner ear with oral administration of this antioxidant supplemented diet supports the possibility of translation to human patients and suggests an opportunity to evaluate antioxidant protection in mouse models of oxidative stress-related disease and pathology.
AB - Oxidative stress has been implicated broadly as a cause of cell death and neural degeneration in multiple disease conditions; however, the evidence for successful intervention with dietary antioxidant manipulations has been mixed. In this study, we investigated the potential for protection of cells in the inner ear using a dietary supplement with multiple antioxidant components, which were selected for their potential interactive effectiveness. Protection against permanent threshold shift (PTS) was observed in CBA/J mice maintained on a diet supplemented with a combination of β-carotene, vitamins C and E, and magnesium when compared with PTS in control mice maintained on a nutritionally complete control diet. Although hair cell survival was not enhanced, noise-induced loss of type II fibrocytes in the lateral wall was significantly reduced (P < 0.05), and there was a trend toward less noise-induced loss in strial cell density in animals maintained on the supplemented diet. Taken together, our data suggest that prenoise oral treatment with the high-nutrient diet can protect cells in the inner ear and reduce PTS in mice. The demonstration of functional and morphologic preservation of cells in the inner ear with oral administration of this antioxidant supplemented diet supports the possibility of translation to human patients and suggests an opportunity to evaluate antioxidant protection in mouse models of oxidative stress-related disease and pathology.
UR - http://www.scopus.com/inward/record.url?scp=79959755331&partnerID=8YFLogxK
U2 - 10.1016/j.trsl.2011.02.006
DO - 10.1016/j.trsl.2011.02.006
M3 - Article
C2 - 21708355
AN - SCOPUS:79959755331
VL - 158
SP - 38
EP - 53
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 1
ER -