Nucleotide inhibition of adenyl cyclase activity in fat cell membranes

Philip E. Cryer; Leonard Jarett; David M. Kipnis

doi:10.1016/0304-4165(69)90323-7

Nucleotide inhibition of adenyl cyclase activity in fat cell membranes

Philip E. Cryer
, Leonard Jarett
, David M. Kipnis

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

A reproducible assay for adenyl cyclase activity in an isolated fat cell membrane preparation is described. Enzyme activity was stimulated by epinephrine and sodium fluoride, but not affected by insulin. GTP, GDP, GMP, CTP and UTP inhibited the adenyl cyclase activity of the fat cell membrane, but guanosine and cyclic GMP had no effect. In contrast to the inhibitory effect on the fat cell membrane enzyme, GTP did not affect either adrenal or kidney adenyl cyclase activity.

Original language	English
Pages (from-to)	586-590
Number of pages	5
Journal	BBA - General Subjects
Volume	177
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(69)90323-7
State	Published - May 6 1969

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10.1016/0304-4165(69)90323-7

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title = "Nucleotide inhibition of adenyl cyclase activity in fat cell membranes",

abstract = "A reproducible assay for adenyl cyclase activity in an isolated fat cell membrane preparation is described. Enzyme activity was stimulated by epinephrine and sodium fluoride, but not affected by insulin. GTP, GDP, GMP, CTP and UTP inhibited the adenyl cyclase activity of the fat cell membrane, but guanosine and cyclic GMP had no effect. In contrast to the inhibitory effect on the fat cell membrane enzyme, GTP did not affect either adrenal or kidney adenyl cyclase activity.",

author = "Cryer, \{Philip E.\} and Leonard Jarett and Kipnis, \{David M.\}",

note = "Funding Information: This work was supported by U.S. Public Health Service Grants AM-I92I and AM-I1892 and AM-o5 027. L.J. is a John and Mary Markle Scholar in Medical Science.",

year = "1969",

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doi = "10.1016/0304-4165(69)90323-7",

language = "English",

volume = "177",

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journal = "BBA - General Subjects",

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TY - JOUR

T1 - Nucleotide inhibition of adenyl cyclase activity in fat cell membranes

AU - Cryer, Philip E.

AU - Jarett, Leonard

AU - Kipnis, David M.

N1 - Funding Information: This work was supported by U.S. Public Health Service Grants AM-I92I and AM-I1892 and AM-o5 027. L.J. is a John and Mary Markle Scholar in Medical Science.

PY - 1969/5/6

Y1 - 1969/5/6

N2 - A reproducible assay for adenyl cyclase activity in an isolated fat cell membrane preparation is described. Enzyme activity was stimulated by epinephrine and sodium fluoride, but not affected by insulin. GTP, GDP, GMP, CTP and UTP inhibited the adenyl cyclase activity of the fat cell membrane, but guanosine and cyclic GMP had no effect. In contrast to the inhibitory effect on the fat cell membrane enzyme, GTP did not affect either adrenal or kidney adenyl cyclase activity.

AB - A reproducible assay for adenyl cyclase activity in an isolated fat cell membrane preparation is described. Enzyme activity was stimulated by epinephrine and sodium fluoride, but not affected by insulin. GTP, GDP, GMP, CTP and UTP inhibited the adenyl cyclase activity of the fat cell membrane, but guanosine and cyclic GMP had no effect. In contrast to the inhibitory effect on the fat cell membrane enzyme, GTP did not affect either adrenal or kidney adenyl cyclase activity.

UR - https://www.scopus.com/pages/publications/0014665676

U2 - 10.1016/0304-4165(69)90323-7

DO - 10.1016/0304-4165(69)90323-7

M3 - Article

C2 - 4306842

AN - SCOPUS:0014665676

SN - 0304-4165

VL - 177

SP - 586

EP - 590

JO - BBA - General Subjects

JF - BBA - General Subjects

IS - 3

ER -

Nucleotide inhibition of adenyl cyclase activity in fat cell membranes

Abstract

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