TY - JOUR
T1 - Nucleotide inhibition of adenyl cyclase activity in fat cell membranes
AU - Cryer, Philip E.
AU - Jarett, Leonard
AU - Kipnis, David M.
N1 - Funding Information:
This work was supported by U.S. Public Health Service Grants AM-I92I and AM-I1892 and AM-o5 027. L.J. is a John and Mary Markle Scholar in Medical Science.
PY - 1969/5/6
Y1 - 1969/5/6
N2 - A reproducible assay for adenyl cyclase activity in an isolated fat cell membrane preparation is described. Enzyme activity was stimulated by epinephrine and sodium fluoride, but not affected by insulin. GTP, GDP, GMP, CTP and UTP inhibited the adenyl cyclase activity of the fat cell membrane, but guanosine and cyclic GMP had no effect. In contrast to the inhibitory effect on the fat cell membrane enzyme, GTP did not affect either adrenal or kidney adenyl cyclase activity.
AB - A reproducible assay for adenyl cyclase activity in an isolated fat cell membrane preparation is described. Enzyme activity was stimulated by epinephrine and sodium fluoride, but not affected by insulin. GTP, GDP, GMP, CTP and UTP inhibited the adenyl cyclase activity of the fat cell membrane, but guanosine and cyclic GMP had no effect. In contrast to the inhibitory effect on the fat cell membrane enzyme, GTP did not affect either adrenal or kidney adenyl cyclase activity.
UR - http://www.scopus.com/inward/record.url?scp=0014665676&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(69)90323-7
DO - 10.1016/0304-4165(69)90323-7
M3 - Article
C2 - 4306842
AN - SCOPUS:0014665676
SN - 0304-4165
VL - 177
SP - 586
EP - 590
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 3
ER -