Nuclear localization of the DOCK180/ELMO complex

Jinhu Yin, Lisa Haney, Scott Walk, Sharleen Zhou, Kodi S. Ravichandran, Weidong Wang

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


DOCK180 protein plays a key role during development, cell motility, and phagocytosis. It forms a complex with another protein ELMO, and this complex acts as a guanine nucleotide exchange factor (GEF) for Rac. However, DOCK180-containing complexes have not been purified by unbiased biochemical approaches, and the nature and subcellular localization of these complexes remain unclear. Here, we show that a large fraction of endogenous DOCK180 is present as a 700kDa nuclear complex with ELMO proteins. In addition, this nuclear DOCK180/ELMO complex has functional Rac-GEF activity. Furthermore, endogenous DOCK180 could be found in complexes with different ELMO isoforms (ELMO1, 2 or 3) in different cell lines, depending on the ELMO isoforms expressed. These studies suggest that DOCK180 may associate with different ELMO proteins to form cell-type specific complexes and may have functions in both the nucleus and the cytoplasm.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Sep 1 2004


  • DOCK180
  • ELMO
  • Guanine nucleotide exchange factor
  • Molecular complex
  • Nuclear localization


Dive into the research topics of 'Nuclear localization of the DOCK180/ELMO complex'. Together they form a unique fingerprint.

Cite this