TY - JOUR
T1 - Nuclear localization of the DOCK180/ELMO complex
AU - Yin, Jinhu
AU - Haney, Lisa
AU - Walk, Scott
AU - Zhou, Sharleen
AU - Ravichandran, Kodi S.
AU - Wang, Weidong
N1 - Funding Information:
We thank Dr. Matsuda for providing anti-DOCK180 antibody. We thank National Cell Culture Center for providing cultured cells. This work was supported by grants from Ellison Medical Foundation and Rett syndrome Research Foundation (W.W.) and by grants from the NIGMS (to K.S.R.).
PY - 2004/9/1
Y1 - 2004/9/1
N2 - DOCK180 protein plays a key role during development, cell motility, and phagocytosis. It forms a complex with another protein ELMO, and this complex acts as a guanine nucleotide exchange factor (GEF) for Rac. However, DOCK180-containing complexes have not been purified by unbiased biochemical approaches, and the nature and subcellular localization of these complexes remain unclear. Here, we show that a large fraction of endogenous DOCK180 is present as a 700kDa nuclear complex with ELMO proteins. In addition, this nuclear DOCK180/ELMO complex has functional Rac-GEF activity. Furthermore, endogenous DOCK180 could be found in complexes with different ELMO isoforms (ELMO1, 2 or 3) in different cell lines, depending on the ELMO isoforms expressed. These studies suggest that DOCK180 may associate with different ELMO proteins to form cell-type specific complexes and may have functions in both the nucleus and the cytoplasm.
AB - DOCK180 protein plays a key role during development, cell motility, and phagocytosis. It forms a complex with another protein ELMO, and this complex acts as a guanine nucleotide exchange factor (GEF) for Rac. However, DOCK180-containing complexes have not been purified by unbiased biochemical approaches, and the nature and subcellular localization of these complexes remain unclear. Here, we show that a large fraction of endogenous DOCK180 is present as a 700kDa nuclear complex with ELMO proteins. In addition, this nuclear DOCK180/ELMO complex has functional Rac-GEF activity. Furthermore, endogenous DOCK180 could be found in complexes with different ELMO isoforms (ELMO1, 2 or 3) in different cell lines, depending on the ELMO isoforms expressed. These studies suggest that DOCK180 may associate with different ELMO proteins to form cell-type specific complexes and may have functions in both the nucleus and the cytoplasm.
KW - DOCK180
KW - ELMO
KW - Guanine nucleotide exchange factor
KW - Molecular complex
KW - Nuclear localization
UR - http://www.scopus.com/inward/record.url?scp=3543034057&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2004.05.014
DO - 10.1016/j.abb.2004.05.014
M3 - Article
C2 - 15288806
AN - SCOPUS:3543034057
SN - 0003-9861
VL - 429
SP - 23
EP - 29
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -