TY - JOUR
T1 - Nuclear and mitochondrial DNA polymorphisms in three mitotic parthenogenetic Meloidogyne spp.
AU - Dautova, Makedonka
AU - Overmars, Hein
AU - Bakker, Jaap
AU - Smant, Geert
AU - Gommers, Fred J.
N1 - Funding Information:
We gratefully acknowledge Dr. G. Karssen (Plant Protection Service, Wageningen, the Netherlands) and J. van Bezooijen (Laboratory of Nematology) for providing the nematode cultures. We are grateful to J. Roosien (Laboratory of Nematology) and M. Jonker (Laboratory of Nematology) for helping and providing advises in statistical analysis.
PY - 2002
Y1 - 2002
N2 - In order to expand our understanding of the genetics of root-knot nematodes, variation in nuclear DNA and mitochondrial DNA in Meloidogyne incognita, M. arenaria and M. javanica was investigated. Despite the obligate mitotic parthenogenetic mode of reproduction, a large number of AFLP polymorphisms were observed among all 16 populations studied. Both UPGMA and principle coordinate analyses revealed three distinct groups that corresponded with the respective species identities of the 16 populations. M. incognita was genetically most distinct. Amplification of 63-bp tandem repeats (TR) in mtDNA from single individuals enabled the calculation of diversity measures at three hierarchical levels: within individuals, among individuals of a single population and among populations. For all three species, the highest diversity was observed within individuals explaining 43-65% of the total diversity. Many individuals contained more than one mtDNA size variant. M. incognita harboured the most heteroplasmic individuals and was the most homogenous at the population level. Only 13% of the total diversity was observed among populations, while this figure was 35% for M. arenaria. Both TR and AFLP data showed that M. arenaria is the most heterogeneous species. The comparison of the genetic distances based on AFLPs and mtDNA size variants revealed a significant correlation for the six M. arenaria populations, whereas no consistent correlation was observed for the populations of the other two species.
AB - In order to expand our understanding of the genetics of root-knot nematodes, variation in nuclear DNA and mitochondrial DNA in Meloidogyne incognita, M. arenaria and M. javanica was investigated. Despite the obligate mitotic parthenogenetic mode of reproduction, a large number of AFLP polymorphisms were observed among all 16 populations studied. Both UPGMA and principle coordinate analyses revealed three distinct groups that corresponded with the respective species identities of the 16 populations. M. incognita was genetically most distinct. Amplification of 63-bp tandem repeats (TR) in mtDNA from single individuals enabled the calculation of diversity measures at three hierarchical levels: within individuals, among individuals of a single population and among populations. For all three species, the highest diversity was observed within individuals explaining 43-65% of the total diversity. Many individuals contained more than one mtDNA size variant. M. incognita harboured the most heteroplasmic individuals and was the most homogenous at the population level. Only 13% of the total diversity was observed among populations, while this figure was 35% for M. arenaria. Both TR and AFLP data showed that M. arenaria is the most heterogeneous species. The comparison of the genetic distances based on AFLPs and mtDNA size variants revealed a significant correlation for the six M. arenaria populations, whereas no consistent correlation was observed for the populations of the other two species.
KW - AFLP
KW - Genetic variations
KW - Plant-parasitic nematodes
KW - Root-knot nematodes
KW - Tandem repeats
UR - http://www.scopus.com/inward/record.url?scp=0036098265&partnerID=8YFLogxK
U2 - 10.1023/A:1015151220460
DO - 10.1023/A:1015151220460
M3 - Article
AN - SCOPUS:0036098265
VL - 108
SP - 209
EP - 220
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
SN - 0929-1873
IS - 3
ER -