Nrl-Cre transgenic mouse mediates loxP recombination in developing rod photoreceptors

Diana S. Brightman, David Razafsky, Chloe Potter, Didier Hodzic, Shiming Chen

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The developing mouse retina is a tractable model for studying neurogenesis and differentiation. Although transgenic Cre mouse lines exist to mediate conditional genetic manipulations in developing mouse retinas, none of them act specifically in early developing rods. For conditional genetic manipulations of developing retinas, a Nrl-Cre mouse line in which the Nrl promoter drives expression of Cre in rod precursors was created. The results showed that Nrl-Cre expression was specific to the retina where it drives rod-specific recombination with a temporal pattern similar to endogenous Nrl expression during retinal development. This Nrl-Cre transgene does not negatively impact retinal structure and function. Taken together, the data suggested that the Nrl-Cre mouse line was a valuable tool to drive Cre-mediated recombination specifically in developing rods.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalGenesis
Volume54
Issue number3
DOIs
StatePublished - Mar 1 2016

Keywords

  • Differentiation
  • Neuronal development
  • Retina-specific Cre
  • Transgenic

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