Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa

Milan Kojic; Branko Jovcic; Alessandro Vindigni; Federico Odreman; Vittorio Venturi

doi:10.1016/j.femsle.2005.04.003

Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa

Milan Kojic, Branko Jovcic, Alessandro Vindigni, Federico Odreman, Vittorio Venturi

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N _2-4 GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies have indicated PsrA as a global regulator.

Original language	English
Pages (from-to)	175-181
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	246
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2005.04.003
State	Published - May 15 2005

Keywords

PsrA regulon
Stationary phase

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10.1016/j.femsle.2005.04.003

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title = "Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa",

abstract = " The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N 2-4 GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies have indicated PsrA as a global regulator.",

keywords = "PsrA regulon, Stationary phase",

author = "Milan Kojic and Branko Jovcic and Alessandro Vindigni and Federico Odreman and Vittorio Venturi",

note = "Funding Information: We are grateful to Rodolfo Garcia Carlos for help in preparing 2-D SDS–PAGE electrophoresis and for offering us the use of his laboratory facilities. We would also like to thank Kristian Vlahovicek for computer assistance. This work was funded by the ICGEB Collaborative Research Program, grant CRP/YUG02-01, and partially supported by Ministry for Science and Environmental Protection of Serbia, grant No. 1442. ",

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AU - Kojic, Milan

AU - Jovcic, Branko

AU - Vindigni, Alessandro

AU - Odreman, Federico

AU - Venturi, Vittorio

N1 - Funding Information: We are grateful to Rodolfo Garcia Carlos for help in preparing 2-D SDS–PAGE electrophoresis and for offering us the use of his laboratory facilities. We would also like to thank Kristian Vlahovicek for computer assistance. This work was funded by the ICGEB Collaborative Research Program, grant CRP/YUG02-01, and partially supported by Ministry for Science and Environmental Protection of Serbia, grant No. 1442.

PY - 2005/5/15

Y1 - 2005/5/15

N2 - The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N 2-4 GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies have indicated PsrA as a global regulator.

AB - The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N 2-4 GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies have indicated PsrA as a global regulator.

KW - PsrA regulon

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Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa

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