Novel protein identification methods for biomarker discovery via a proteomic analysis of periodontally healthy and diseased gingival crevicular fluid samples

Richard C. Baliban, Dimitra Sakellari, Zukui Li, Peter A. Dimaggio, Benjamin A. Garcia, Christodoulos A. Floudas

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Aim To identify possible novel biomarkers in gingival crevicular fluid (GCF) samples from chronic periodontitis (CP) and periodontally healthy individuals using high-throughput proteomic analysis. Materials and Methods Gingival crevicular fluid samples were collected from 12 CP and 12 periodontally healthy subjects. Samples were trypically digested with trypsin, eluted using high-performance liquid chromatography, and fragmented using tandem mass spectrometry (MS/MS). MS/MS spectra were analysed using PILOT-PROTEIN to identify all unmodified proteins within the samples. Results Using the database derived from Homo sapiens taxonomy and all bacterial taxonomies, 432 human (120 new) and 30 bacterial proteins were identified. The human proteins, angiotensinogen, clusterin and thymidine phosphorylase were identified as biomarker candidates based on their high-scoring only in samples from periodontal health. Similarly, neutrophil defensin-1, carbonic anhydrase-1 and elongation factor-1 gamma were associated with CP. Candidate bacterial biomarkers include 33 kDa chaperonin, iron uptake protein A2 and phosphoenolpyruvate carboxylase (health-associated) and ribulose biphosphate carboxylase, a probable succinyl-CoA:3-ketoacid-coenzyme A transferase, or DNA-directed RNA polymerase subunit beta (CP-associated). Most of these human and bacterial proteins have not been previously evaluated as biomarkers of periodontal conditions and require further investigation. Conclusions The proposed methods for large-scale comprehensive proteomic analysis may lead to the identification of novel biomarkers of periodontal health or disease.

Original languageEnglish
Pages (from-to)203-212
Number of pages10
JournalJournal of Clinical Periodontology
Volume39
Issue number3
DOIs
StatePublished - Mar 2012

Keywords

  • biomarkers
  • gingival crevicular fluid
  • periodontitis
  • proteomic analysis
  • tandem mass spectrometry

Fingerprint

Dive into the research topics of 'Novel protein identification methods for biomarker discovery via a proteomic analysis of periodontally healthy and diseased gingival crevicular fluid samples'. Together they form a unique fingerprint.

Cite this