TY - JOUR
T1 - Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas
AU - Shen-Ong, Grace L.C.
AU - Keath, Elizabeth J.
AU - Piccoli, Steven P.
AU - Cole, Michael D.
N1 - Funding Information:
We would like to thank Mark Groudine for providing the chicken myc clone, Michael Green and Robert Roeder for the generous gift of solid tumors and James Swierkosz for help in preparing B cells. We thank Stuart Kim and Leroy Hood for providing the germline C, clone and for communicating results prior to publication. This work was supported by grants from the American Cancer Society and the Sklarow Memorial Fund. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC. Section 1734 solely to indicate this fact.
PY - 1982/12
Y1 - 1982/12
N2 - We have found that the myc oncogene has been modified by abortive recombination with the α heavy-chain immunoglobulin constant-region (Cα) gene in five different mouse plasmacytoma lines. Recombination occurred approximately 0.8-2.0 kb to the 5′ side of two distinct coding regions, defined by sequence homology between the chicken cellular and plasmacytoma myc genes. The myc and Cα genes were always in opposite transcriptional orientation, with the recombination site within the Cα switch region sequences. DNA recombination was found to correlate with the production of a novel 2.1 kb species of myc RNA that was 0.4 kb shorter than the normal cellular transcript. No elevated levels of myc RNA were evident, suggesting that DNA rearrangements have altered the myc oncogene product. This oncogene activation corresponds to the chromosomal translocations found in nearly all plasmacytomas.
AB - We have found that the myc oncogene has been modified by abortive recombination with the α heavy-chain immunoglobulin constant-region (Cα) gene in five different mouse plasmacytoma lines. Recombination occurred approximately 0.8-2.0 kb to the 5′ side of two distinct coding regions, defined by sequence homology between the chicken cellular and plasmacytoma myc genes. The myc and Cα genes were always in opposite transcriptional orientation, with the recombination site within the Cα switch region sequences. DNA recombination was found to correlate with the production of a novel 2.1 kb species of myc RNA that was 0.4 kb shorter than the normal cellular transcript. No elevated levels of myc RNA were evident, suggesting that DNA rearrangements have altered the myc oncogene product. This oncogene activation corresponds to the chromosomal translocations found in nearly all plasmacytomas.
UR - http://www.scopus.com/inward/record.url?scp=0020355993&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(82)90137-4
DO - 10.1016/0092-8674(82)90137-4
M3 - Article
C2 - 6819085
AN - SCOPUS:0020355993
SN - 0092-8674
VL - 31
SP - 443
EP - 452
JO - Cell
JF - Cell
IS - 2 PART 1
ER -