Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas

Grace L.C. Shen-Ong, Elizabeth J. Keath, Steven P. Piccoli, Michael D. Cole

Research output: Contribution to journalArticle

259 Scopus citations

Abstract

We have found that the myc oncogene has been modified by abortive recombination with the α heavy-chain immunoglobulin constant-region (Cα) gene in five different mouse plasmacytoma lines. Recombination occurred approximately 0.8-2.0 kb to the 5′ side of two distinct coding regions, defined by sequence homology between the chicken cellular and plasmacytoma myc genes. The myc and Cα genes were always in opposite transcriptional orientation, with the recombination site within the Cα switch region sequences. DNA recombination was found to correlate with the production of a novel 2.1 kb species of myc RNA that was 0.4 kb shorter than the normal cellular transcript. No elevated levels of myc RNA were evident, suggesting that DNA rearrangements have altered the myc oncogene product. This oncogene activation corresponds to the chromosomal translocations found in nearly all plasmacytomas.

Original languageEnglish
Pages (from-to)443-452
Number of pages10
JournalCell
Volume31
Issue number2 PART 1
DOIs
StatePublished - Dec 1982
Externally publishedYes

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